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US 11,434,491 B2Granted
Compositions and methods for gene editing
AssigneeThe Regents of the University of California
InventorsJin Chen, Luke Gilbert, James Nunez, Jonathan Weissman
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Abstract
Provided herein are, inter alia, compositions and methods for manipulation of genomes of living organisms.
Claims41
A fusion protein comprising, from N-terminus to C-terminus:
- (i) a DNA methyltransferase domain, a nuclease-deficient RNA-guided DNA endonuclease enzyme, and a Krüppel associated box domain;
- or
- (ii) a Krüppel associated box domain, a nuclease-deficient RNA-guided DNA endonuclease enzyme, and a DNA methyltransferase domain.
A fusion protein comprising, a DNA methyltransferase domain, a nuclease-deficient RNA-guided DNA endonuclease enzyme, and a Krüppel associated box domain;
- wherein
- (1) the nuclease-deficient RNA-guided DNA endonuclease enzyme is dCas9 which comprises an amino acid sequence having at least 85% sequence identity to SEQ ID NO:23;
- ddCas12a which comprises an amino acid sequence having at least 85% sequence identity to SEQ ID NO:34;
- dLbCfp1 which comprises an amino acid sequence having at least 85% sequence identity to SEQ ID NO:35;
- or dFnCfp1 which comprises an amino acid sequence having at least 85% sequence identity to SEQ ID NO:36;
- (2) the Krüppel associated box domain comprises an amino acid sequence having at least 85% sequence identity to SEQ ID NO:16;
- and
- (3) the DNA methyltransferase domain comprises a Dnmt3A protein, a Dnmt3L protein, or a Dnmt3A protein and a Dnmt3L protein;
- wherein the Dnmt3A protein comprises an amino acid sequence with at least 85% sequence identity to SEQ ID NO:26, and the Dnmt3L protein comprises an amino acid sequence with at least 85% sequence identity to SEQ ID NO:28.
6 more elements
Dependencies are inferred from the claim text.