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US 11,434,476 B2Granted
Methods of editing DNA methylation
AssigneeWhitehead Institute for Biomedical Research
InventorsRudolf Jaenisch, X Shawn Liu, Hao Wu
Life of this patent
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Abstract
The invention relates to methods of modifying DNA methylation by contacting a catalytically inactive site specific nuclease fused to an effector domain having methylation or demethylation activity and one or more guide sequences.
Claims16
A method of modulating the methylation of one or more CTCF binding sites in a cell comprising introducing into the cell
- a. a catalytically inactive site specific nuclease fused to an effector domain having methylation activity, wherein the effector domain comprises Dnmt3a;
- and
- b. one or more guide sequences or a nucleic acid that encodes the one or more guide sequences, wherein the one or more guide sequences target the CTCF binding sites, thereby modulating the methylation of one or more genomic sequences in the cell.
A method of modulating the methylation of one or more enhancers of MyoD in a cell comprising introducing into the cell
- a. a catalytically inactive site specific nuclease fused to an effector domain having demethylation activity, wherein the effector domain comprises Tet1;
- and
- b. one or more guide sequences or a nucleic acid that encodes one or more guide sequences, wherein the one or more guide sequences target the enhancers of MyoD, thereby modulating the methylation of one or more genomic sequences in the cell.
Dependencies are inferred from the claim text.