Optimized Zika virus envelope gene and expression thereof
AssigneeHawaii Biotech, Inc.
InventorDavid E. Clements
Life of this patent
Estimating the term…
Abstract
The present invention is directed to the expression and secretion the Zika virus envelope protein. Elements of the pre-membrane and envelope sequence have been modified to enhance the expression of the envelope protein as a secreted product in the culture medium of transformed insect cell lines. The expressed and purified product is suitable as a vaccine antigen.
Claims7
An expression vector comprising a DNA sequence encoding Zika virus pre-membrane and envelope protein, wherein expression of the DNA sequence results in secretion of a soluble envelope protein in the culture medium, wherein the DNA sequence comprises SEQ ID NO:1.
An expression vector comprising:
- a DNA sequence encoding Zika virus pre-membrane and envelope protein, wherein expression of the DNA sequence results in secretion of a soluble envelope protein in the culture medium, and
- an expression cassette that comprises SEQ ID NO:7.
An expression vector for expression and secretion of heterologous proteins in cultured insect cells, wherein the expression vector comprises the expression cassette shown in SEQ ID NO:7.
Dependencies are inferred from the claim text.