# US 11,433,123 B2 · Sustained release cowpea mosaic virus or virus-like particle therapeutic constructs for the treatment of cancer

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- Number: US 11,433,123 B2
- Document ID: US11433123B2
- Status: Granted
- Kind: B2
- Application number: 16/319,765
- Filed: 2017-07-21
- Published: 2021-09-09
- Granted: 2022-09-06
- Earliest claimed: 2016-07-21
- Assignees: Case Western Reserve University
- Applicants: Case Western Reserve University
- Inventors: Nicole F. Steinmetz; Frank Alexander Veliz; Brylee David B. Tiu
- Web page: https://patentgrep.com/patent/US11433123B2

## CPC

- A61K 47/6901: {Conjugates being cells, cell fragments, viruses, ghosts, red blood cells or viral vectors}
- A61K 39/0011: {Cancer antigens}
- A61K 39/385: Haptens or antigens, bound to carriers
- A61K 39/39: characterised by the immunostimulating additives, e.g. chemical adjuvants
- A61P 35/00: Antineoplastic agents
- A61K 2039/5258: {Virus-like particles}
- A61K 2039/55555: {Liposomes; Vesicles, e.g. nanoparticles; Spheres, e.g. nanospheres; Polymers}
- A61K 2039/585: {wherein the target is cancer}
- A61K 2039/622: {non-covalent binding}
- A61K 2039/645: {Dendrimers; Multiple antigen peptides}
- B82Y 5/00: Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
- C12N 2770/18034: Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- B82Y: SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

A method of treating cancer in a subject in need thereof includes administering in situ to cancer cells of the subject a nanoparticle construct that includes a plurality of cowpea mosaic virus or virus-like particles electrostatically coupled to a plurality of G4 dendrimers having a different surface charge than the cowpea mosaic virus or virus-like particles. The nanoparticle construct upon delivery to a subject can provide a sustained release of the cowpea mosaic virus or virus-like particles and/or G4 dendrimers to a cell or tissue of the subject. The cancer selected from the group consisting of melanoma, breast cancer, colon cancer, lung cancer, and ovarian cancer.

## Claims

### Claim 1

A method of treating cancer in a subject in need thereof, the method comprising:
administering in situ to cancer cells of the subject a nanoparticle construct, the nanoparticle construct comprising:
a plurality of cowpea mosaic virus or virus-like particles and
a plurality of G4 dendrimers having a different surface charge than the cowpea mosaic virus or virus-like particles electrostatically coupled to the cowpea mosaic virus or virus-like particles, the nanoparticle construct upon in situ delivery to the subject providing a sustained release of the cowpea mosaic virus or virus-like particles and/or nanoparticles to the cancer cells, wherein the cancer is selected from the group consisting of melanoma, breast cancer, colon cancer, lung cancer, and ovarian cancer.

### Claim 2 (depends on 1)

The method of claim 1, the nanoparticle construct having a hydrodynamic radius of about 100 nm to about two microns.

### Claim 3 (depends on 1)

The method of claim 1, the nanoparticle construct upon administration to a subject disassembles in a sustained manner at physiological pH.

### Claim 4 (depends on 1)

The method of claim 1, wherein the cowpea mosaic virus or virus-like particle is a cowpea mosaic virus-like particle.

### Claim 5 (depends on 1)

The method of claim 1, the nanoparticle construct comprising alternating electrostatically coupled layers of the cowpea mosaic virus or virus-like particles and the G4 dendrimers.

### Claim 6 (depends on 5)

The method of claim 5, wherein the cowpea mosaic virus or virus-like particle is loaded with or bonded to a cargo molecule.

### Claim 7 (depends on 6)

The method of claim 6, wherein the cargo molecule is an anticancer agent.

### Claim 8 (depends on 1)

The method of claim 1, wherein the nanoparticle construct is administered proximal to a tumor in the subject.

### Claim 9 (depends on 1)

The method of claim 1, wherein the cancer is metastatic cancer.

### Claim 10 (depends on 1)

The method of claim 1, wherein the cancer is ovarian cancer.