# US 11,433,019 B2 · Ganoderma lucidum extract-preparing method using eco-friendly extraction technique, Ganoderma lucidum extract prepared thereby, and anti-aging cosmetic composition comprising same extract

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- Number: US 11,433,019 B2
- Document ID: US11433019B2
- Status: Granted
- Kind: B2
- Application number: 16/651,966
- Filed: 2018-09-20
- Published: 2020-08-13
- Granted: 2022-09-06
- Earliest claimed: 2017-09-28
- Assignees: Amorepacific Corporation
- Applicants: Amorepacific Corporation
- Inventors: Jin Sup Shim; Eun Jung Lee; Nok Hyun Park; So Woong Choi; Yong Jin Kim
- Web page: https://patentgrep.com/patent/US11433019B2

## CPC

- A61K 8/9728: Fungi, e.g. yeasts
- A61K 8/31: Hydrocarbons
- A61K 8/63: Steroids; Derivatives thereof
- A61K 8/738: {Cyclodextrins}
- A61Q 19/08: Anti-ageing preparations
- A61K 2800/57: Compounds covalently linked to a(n inert) carrier molecule, e.g. conjugates, pro-fragrances
- A61K 2800/59: Mixtures
- A61K 2800/82: Preparation or application process involves sonication or ultrasonication
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61Q: SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS

## Abstract

A method for preparing Ganoderma lucidum extract using an eco-friendly extraction technique is disclosed. A Ganoderma lucidum extract prepared thereby and a cosmetic composition containing the extract, and their uses are disclosed. The extraction technique use an aqueous cyclodextrin solution alone or in combination with ultrasonic extraction to afford a Ganoderma lucidum extract containing a high concentration of the effective ingredient triterpenoid.

## Claims

### Claim 1

A method for preparing a Ganoderma lucidum extract, the method comprising extracting Ganoderma lucidum with an aqueous cyclodextrin solution to form a clathrate of a triterpenoid component of the Ganoderma lucidum and a cyclodextrin of the aqueous cyclodextrin solution,
wherein the Ganoderma lucidum extract contains triterpenoids of Ganoderma lucidum, and
wherein the aqueous cyclodextrin solution contains the cyclodextrin in a concentration ranging from 0.01 to 30% by weight based on total volume of the aqueous cyclodextrin solution.

### Claim 2 (depends on 1)

The method of claim 1, further comprising an ultrasonic treatment during the extracting step of forming the clathrate of the cyclodextrin and the triterpenoid component of the Ganoderma lucidum.

### Claim 3 (depends on 1)

The method of claim 1, wherein the cyclodextrin in the aqueous cyclodextrin solution is a mixture of α-cyclodextrin, β-cyclodextrin, and γ-cyclodextrin.

### Claim 4 (depends on 1)

The method of claim 1, wherein the aqueous cyclodextrin solution comprises a mixture of βcyclodextrin and γ-cyclodextrin at a weight ratio of 1:1; or a mixture of α-cyclodextrin, β-cyclodextrin, and γ-cyclodextrin at a weight ratio of 1:1 to 2:0.5 to 2.

### Claim 5 (depends on 1)

The method of claim 1, wherein temperature of the extraction is 15° C. to 100° C.

### Claim 6 (depends on 1)

The method of claim 1, wherein duration of the extraction is 10 minutes to 24 hours.

### Claim 7 (depends on 2)

The method of claim 2, wherein temperature of the extraction during the ultrasonic treatment is 15° C. to 35° C., and duration of the extraction is 10 minutes to 120 minutes.