# US 11,432,566 B2 · Feed composition for preventing or treating acute hepatopancreatic necrosis disease (AHPND) or white spot syndrome (WSS), comprising a Bacillus subtilis strain, a Bacillus pumilus strain, and a Bacillus licheniformis strain as active ingredients

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- Number: US 11,432,566 B2
- Document ID: US11432566B2
- Status: Granted
- Kind: B2
- Application number: 16/349,838
- Filed: 2018-12-28
- Published: 2020-10-08
- Granted: 2022-09-06
- Earliest claimed: 2017-12-29
- Assignees: Cj Cheildejang Corporation
- Applicants: CJ Cheiljedang Corporation
- Inventors: Ji Eun Kim; Sung Hun Kim; Jae Won Kim; Seo Hyung Woo; Jongsu Eun; Hayun Jo; Jee Eun Han
- Web page: https://patentgrep.com/patent/US11432566B2

## CPC

- A23K 10/16: Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions (A23K10/12 takes precedence)
- A23K 10/18: of live microorganisms
- A23K 50/80: for aquatic animals, e.g. fish, crustaceans or molluscs
- A61K 35/741: Probiotics (probiotic yeast, e.g. saccharomyces A61K36/06)
- A61K 35/742: Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
- A61K 39/07: Bacillus
- A61K 39/107: {Vibrio}
- A61P 1/16: for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
- A61P 1/18: for pancreatic disorders, e.g. pancreatic enzymes
- A23K: FODDER
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

A feed composition for preventing or treating acute hepatopancreatic necrosis disease (AHPND) or white spot syndrome (WSS), having a Bacillus subtilis (KCCM11143P) strain, a Bacillus pumilus (KCCM11144P) strain, and a Bacillus licheniformis (KCCM11270P) strain; culture media thereof; concentrates thereof; or dry matters thereof as an active ingredient. The feed composition exhibits antibacterial activity against Vibrio parahaemolyticus, which causes shrimp AHPND, and antiviral activity against white spot syndrome virus (WSSV), which causes WSS.

## Claims

### Claim 1

A feed composition or a feed additive for improving the non-specific immune response of a crustacean subject against acute hepatopancreatic necrosis disease (AHPND), the composition or the additive comprising, as active ingredients, 1×104 CFU to 1×1011 CFU per gram of isolated Bacillus strains, wherein the Bacillus strains are Bacillus subtilis deposited as KCCM11143P, Bacillus pumilus deposited as KCCM11144P, and Bacillus licheniformis deposited as KCCM11270P.

### Claim 2 (depends on 1)

The feed composition or the feed additive of claim 1, wherein the AHPND is caused by Vibrio haemolyticus.

### Claim 3 (depends on 1)

The feed composition or the feed additive of claim 1, wherein the crustacean subject is a shrimp.

### Claim 4

A feed composition or a feed additive for improving the non-specific immune response of a crustacean subject against white spot syndrome (WSS), the composition or the additive comprising, as active ingredients, 1×104 CFU to 1×1011 CFU per gram of isolated Bacillus strains, wherein the Bacillus strains are Bacillus subtilis deposited as KCCM11143P, Bacillus pumilus deposited as KCCM11144P, and Bacillus licheniformis deposited as KCCM11270P.

### Claim 5 (depends on 4)

The feed composition or the feed additive of claim 4, wherein the WSS is caused by white spot syndrome virus (WSSV).

### Claim 6 (depends on 4)

The feed composition or the feed additive of claim 4, wherein the crustacean subject is a shrimp.

### Claim 7 (depends on 1)

A method of improving the non-specific immune response of a crustacean subject against acute hepatopancreatic necrosis disease (AHPND) comprising feeding the subject with an effective dose of the feed composition or the feed additive of claim 1.

### Claim 8 (depends on 7)

The method of claim 7, wherein the crustacean subject is a shrimp.

### Claim 9 (depends on 4)

A method of improving the non-specific immune response of a crustacean subject against white spot syndrome (WSS) comprising feeding the subject with an effective dose of the feed composition or the feed additive of claim 4.

### Claim 10 (depends on 9)

The method of claim 9, wherein the crustacean subject is a shrimp.