# US 11,432,474 B2 · Plant cultivation method for increasing phytochemical content > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,432,474 B2 - Document ID: US11432474B2 - Status: Granted - Kind: B2 - Application number: 17/208,989 - Filed: 2021-03-22 - Published: 2021-09-23 - Granted: 2022-09-06 - Earliest claimed: 2020-03-23 - Assignees: Seoul Viosys Co., Ltd. - Applicants: Seoul Viosys Co., Ltd. - Inventors: Se Ryung Kim - Web page: https://patentgrep.com/patent/US11432474B2 ## CPC - A01G 7/045: {with electric lighting} - A01G 9/249: {Lighting means (with special arrangements for promoting growth A01G7/045)} - A01G 22/15: Leaf crops, e.g. lettuce or spinach - A01G: HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING (picking of fruits, vegetables, hops or the like A01D46/00; propagating unicellular algae C12N1/12) ## Abstract A plant cultivation method for increasing the phytochemical content of a Labiatae plant is provided. The plant cultivation method includes cultivating the Labiatae plant in a visible light environment having a dark period and a light period during which the Labiatae plant is exposed to visible light in an alternate manner, and performing treatment with UVB radiation during the light period for at least one day before harvesting. A cumulative dose of the UVB radiation may range from 0.54J/m2 to 4.32 kJ/m2. ## Claims ### Claim 1 A plant cultivation method for increasing content of a phytochemical, the method comprising: cultivating a Labiatae plant in a visible light environment having a dark period and a light period, wherein the Labiatae plant is exposed to visible light during the dark period and during the light period, the dark period being alternate with the light period; performing UV treatment using UVB radiation to the Labiatae plant; and controlling content of a phytochemical in the Labiatae plant based on at least three parameters, wherein the at least three parameters comprise a cumulative dose of the UVB radiation, a duration of the UV treatment prior to harvesting, and a timing of the UV treatment; and wherein the cumulative dose of the UVB radiation ranges from 0.54 J/m2 to 4.32 kJ/m2 and the cumulative dose of the UVB radiation varies dependent upon the duration of the UV treatment. ### Claim 2 (depends on 1) The plant cultivation method according to claim 1, further comprising: setting the light period to 16 hours; and setting the dark period is set to 8 hours. ### Claim 3 (depends on 1) The plant cultivation method according to claim 1, further comprising: applying the visible light with a light source including an LED, wherein applying the visible light further comprises applying mixed light in which red, white, and blue light are mixed in a ratio of 11:4:3. ### Claim 4 (depends on 1) The plant cultivation method according to claim 1, wherein performing the UV treatment further comprises performing the UV treatment using the UVB radiation having an intensity that ranges from 413 kJ/mol to 427 kJ/mol. ### Claim 5 (depends on 1) The plant cultivation method according to claim 1, wherein performing the UV treatment further comprises exposing the Labiatae plant to the UVB radiation for one day before harvesting, the UVB radiation having a cumulative dose of 0.54 J/m2. ### Claim 6 (depends on 5) The plant cultivation method according to claim 5, wherein the phytochemical is rosmarinic acid and the rosmarinic acid content of the Labiatae plant is increased by 73% through the UV treatment. ### Claim 7 (depends on 1) The plant cultivation method according to claim 1, wherein performing the UV treatment further comprises exposing the Labiatae plant to the UVB radiation for 4 days before harvesting with a cumulative dose of the UVB radiation of 2.16 J/m. ### Claim 8 (depends on 7) The plant cultivation method according to claim 7, wherein the phytochemical is flavonol and the flavonol content of the Labiatae plant is increased by 74% through the UV treatment. ### Claim 9 (depends on 7) The plant cultivation method according to claim 7, wherein the phytochemical is rosmarinic acid and the rosmarinic acid content of the Labiatae plant is increased by 51% through the UV treatment. ### Claim 10 (depends on 1) The plant cultivation method according to claim 1, wherein performing the UV treatment further comprises exposing the Labiatae plant to the UVB radiation for four days before harvesting with a cumulative dose of the UVB radiation of 4.32 J/m2. ### Claim 11 (depends on 10) The plant cultivation method according to claim 10, wherein the phytochemical is flavonol and the flavonol content of the Labiatae plant is increased by 81% through the UV treatment. ### Claim 12 (depends on 10) The plant cultivation method according to claim 10, wherein the phytochemical is rosmarinic acid and the rosmarinic acid content of the Labiatae plant is increased by 87% through the UV treatment. ### Claim 13 (depends on 1) The plant cultivation method according to claim 1, wherein cultivating the Labiatae plant further comprises: cultivating the Labiatae plant in the visible light environment for a total of 24 days after transplanting germinated seeds thereof into a hydroponics system; and exposing the Labiatae plant to the visible light with 150 PPFD for the light period for the 24 days of cultivation; and wherein performing the UV treatment further comprises performing the UV treatment during the light period the day before harvesting. ### Claim 14 (depends on 13) The plant cultivation method according to claim 13, wherein the Labiatae plant is perilla. ### Claim 15 (depends on 1) The plant cultivation method according to claim 1, wherein cultivating the Labiatae plant further comprises: cultivating the Labiatae plant in the visible light environment for a total of 36 days after transplanting germinated seeds thereof into a hydroponics system; exposing the Labiatae plant to the visible light with 150 PPFD for the light period for first 7 days of cultivation; exposing the Labiatae plant to the visible light with 268 PPFD for the light period for the other 29 days of cultivation; and performing the UV treatment further comprises performing the UV treatment during the light period once a day, for 4 days before harvesting. ### Claim 16 (depends on 15) The plant cultivation method according to claim 15, wherein the Labiatae plant is sweet basil.