Detection methods employing HCV core lipid and DNA binding domain monoclonal antibodies
AssigneeAbbott Laboratories
InventorsRobert Ziemann, April Ahlberg, David Hawksworth, Bryan Tieman, A. Scott Muerhoff, Christopher Marohnic, Kathy Otis
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Abstract
The present disclosure provides detection methods employing HCV core lipid binding domain and DNA binding domain monoclonal antibodies. In certain embodiments, the lipid binding domain monoclonal antibody recognizes an epitope in amino acids 141 to 161 of HCV core protein.
Claims9
A method for the detection of HCV protein in a test sample comprising:
- (i) contacting a test sample suspected of containing HCV with a first monoclonal antibody, or antigen-binding portion thereof, which specifically binds to an epitope in the DNA binding domain of an HCV core protein to form a first complex between said first monoclonal antibody, or antigen-binding fragment thereof, and said HCV core protein located within said test sample;
- (ii) contacting the first complex formed in step (i) with a second monoclonal antibody comprising a heavy chain variable domain amino acid sequence of SEQ ID NO:
- 100 and a light chain variable domain amino acid sequence of SEQ ID NO:
- 382 and/or a third monoclonal antibody comprising a heavy chain variable domain amino acid sequence of SEQ ID NO:
- 44 and a light chain variable domain amino acid sequence of SEQ ID NO:
- 342, or an antigen-binding fragment thereof, to form a second complex between the second monoclonal antibody and/or the third monoclonal antibody, or antigen-binding fragment thereof, and said first complex formed in step (i), and
- (iii) detecting the second complex formed in step (ii).
A monoclonal antibody comprising a heavy chain variable domain amino acid sequence of SEQ ID NO:
- 100 and a light chain variable domain amino acid sequence of SEQ ID NO:
- 382, which specifically binds to the lipid binding domain of the HCV core antigen.
A monoclonal antibody comprising a heavy chain variable domain amino acid sequence of SEQ ID NO:
- 44 and a light chain variable domain amino acid sequence of SEQ ID NO:
- 342, which specifically binds to the lipid binding domain of the HCV core antigen.
Dependencies are inferred from the claim text.