Methods for identifying and modulating co-occurant cellular phenotypes

A cell functionalizing probe comprising:

• (a) a polyadorned molecule comprising 3 to 5 substituents, comprising;
• (i) a first photoactivated cell-surface reactive group comprising a diazirine or a benzophenone;
• (ii) a first bio-orthogonal reactive group comprising a compound of Formula (I):
• wherein R1 is selected from the group consisting of —H, —X, —(CH2)a—NH-PG1, O—(CH2CH2O)a(CH2)cNH2—PG1, O—(CH2CH2O)a—PG2, —(CH2)a—O-PG2;
• R2 is selected from the group consisting of —C(O)(CH2)aCO2R21, —CO2(CH2CH2O)aCO2R21, —C(O)(CH2)aCONR21, —CO2(CH2CH2O)aCONR21, and —CO(CH2)aNHCO(CH2)aO(CH2CH2O)c(CH2)aNHR21;
• R21 is selected from the group consisting of —H, —O(C1-C6 alkyl), —C1-C8 straight chain alkyl, —C1-C8 branched alkyl, —C2-C8 alkenyl, —C2-C8 alkynyl and —(C1-C6 alkyl)-O—(C1-C6 alkyl);
• PG1 is an amine protecting group or —H;
• PG2 is an alcohol protecting group or —H;
• and
• X is selected from the group consisting of Cl, Br, I, and F;
• a is independently any integer between 0 and 6;
• c is independently any integer between 1 and 6;
• and
• the additional substituents are individually selected from a label, a cell-surface reactive group, or a bio-orthogonal reactive group, and wherein when the photoactivated cell-surface reactive group comprises benzophenone, the polyadorned molecule comprises phenyl, naphthyl, anthracenyl, phenanthrenyl, indanyl, indenyl, dihydroxyaryl or triazine.