Method for improving stem volume growth and biomass production in trees
AssigneeStora Enso OYJ
InventorsJuha Immanen, Yrjö Helariutta, Kaisa Nieminen
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Abstract
The present invention relates to a genetic construct comprising a nucleic acid sequence encoding cytokinin biosynthetic isopentenyl-transferase enzyme (IPT) operable linked to a promoter allowing expression of said nucleic acid sequence in cambial cells. The invention relates also a method for producing a transgenic plant capable of increased biomass production and/or increased stem volume growth compared to wild type plant and a method for improving the production of biomass and/or increased stem volume growth in trees, as well as to a tree that over expresses an endogenous or exogenous nucleic acid sequence encoding IPT in cambial cells and a wood product obtainable from the transgenic tree.
Claims17
A genetic construct comprising or having contained therein a first nucleic acid encoding a polypeptide having cytokinin biosynthetic isopentenyl-transferase enzyme activity operably linked to a second nucleic acid comprising a promoter active in a cambial cell to allow expression of said first nucleic acid in the cambial cell,
- wherein said first nucleic acid has at least about 95% sequence identity to SEQ ID NO:2;
- and
- wherein said second nucleic acid sequence comprises a birch meristem promoter pBpCRE1 comprising a sequence having at least 95 % sequence identity to SEQ ID NO:
- 7.
A genetic construct comprising or having contained therein a first nucleic acid encoding a polypeptide having cytokinin biosynthetic isopentenyl-transferase enzyme activity operably linked to a second nucleic acid comprising a promoter active in a cambial cell to allow expression of said first nucleic acid in the cambial cell,
- wherein said first nucleic acid has at least about 95% sequence identity to SEQ ID NO:2;
- and
- wherein said second nucleic acid sequence comprises a cambial specific promoter comprising a sequence having at least 95% sequence identity to SEQ ID NO:8.
A genetic construct comprising or having contained therein a first nucleic acid encoding a polypeptide having cytokinin biosynthetic isopentenyl-transferase enzyme activity operably linked to a second nucleic acid comprising a promoter active in a cambial cell to allow expression of said first nucleic acid in the cambial cell,
- wherein said first nucleic acid comprises a nucleic acid sequence encoding an amino acid sequence comprising a conserved domain area A, B and/or C, wherein conserved domain A comprises an amino acid sequence having at least 95% sequence identity to SEQ ID NO:3, wherein conserved domain B comprises an amino acid sequence having at least 95% sequence identity to SEQ ID NO:4, and wherein conserved domain C comprises an amino acid sequence having at least 95% sequence identity to SEQ ID NO:5 , and wherein said first nucleic acid sequence encodes an adenosine phosphate-isopentenyltransferase enzyme 7, IPT7 or an adenosine phosphate-isopentenyltransferase enzyme 5, IPT5;
- and
- wherein said second nucleic acid sequence comprises a birch meristem promoter pBpCRE1 comprising a sequence having at least 95% sequence identity to SEQ ID NO:
- 7.
A genetic construct comprising or having contained therein a first nucleic acid encoding a polypeptide having cytokinin biosynthetic isopentenyl-transferase enzyme activity operably linked to a second nucleic acid comprising a promoter active in a cambial cell to allow expression of said first nucleic acid in the cambial cell,
- wherein said first nucleic acid comprises a nucleic acid sequence encoding an amino acid sequence comprising a conserved domain area A, B and/or C, wherein conserved domain A comprises an amino acid sequence having at least 95% sequence identity to SEQ ID NO:3, wherein conserved domain B comprises an amino acid sequence having at least 95% sequence identity to SEQ ID NO:4, and wherein conserved domain C comprises an amino acid sequence having at least 95% sequence identity to SEQ ID NO:5, and wherein said first nucleic acid sequence encodes an adenosine phosphate-isopentenyltransferase enzyme 7, IPT7 or an adenosine phosphate-isopentenyltransferase enzyme 5, IPT5;
- and
- wherein said second nucleic acid sequence comprises a cambial specific promoter comprising a sequence having at least 95% sequence identity to SEQ ID NO:8.
Dependencies are inferred from the claim text.