Immunoassay employing sulfated polysaccharide
AssigneeLsi Medience Corporation
InventorsRyuhei Shimizu, Keiichi Shoji, Xu Zhang
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Abstract
Regardless of the type of specimen, such as a serum or heparinized plasma containing different anticoagulants, which are widely used in general, when a substance to be measured (for example, sIL-2R) in a biological sample is immunologically detected, a measuring method and a kit capable of stably obtaining with high accuracy, unaffected by interfering substances in the specimen, are provided. An immunocomplex between the substance to be measured and an antibody that specifically binds to the substance to be measured is formed in the presence of a sulfated polysaccharide. The kit comprises an antibody that specifically binds to the substance to be measured, and a buffer containing a sulfated polysaccharide.
Claims5
A method of immunologically measuring a substance to be measured in a biological sample, said method comprising:
- providing a sample selected from the group consisting of a blood sample to which heparin is added as an anticoagulant, and a serum sample;
- bringing the sample into contact with an antibody that specifically binds to the substance to be measured in the presence of 0.002% to 0.4% β-cyclodextrin sulfate to form an immunocomplex;
- and
- analyzing the immunocomplex or a signal derived from the immunocomplex.
Dependencies are inferred from the claim text.