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US 11,421,199 B2Granted
Recombinant yeast and use thereof
AssigneesTianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences
InventorsXueli Zhang, Luqi Huang, Zhubo Dai, Dong Wang, Lili Zhang, Juan Guo, Yi Liu
Life of this patent
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Abstract
Provided is a recombinant yeast expressing germacrene A synthetase or a fusion protein thereof, wherein the fusion protein is germacrene A synthetase and farnesyl pyrophosphate synthase. The recombinant yeast improves the yield of germacrene A, and is suitable for the industrialized production of β-elemene and/or germacrene A.
Claims25
A recombinant yeast strain, comprising:
- a fusion protein comprising germacrene A synthetase, farnesyl pyrophosphate synthase and a linker peptide linking the germacrene A synthetase with the farnesyl pyrophosphate synthase, wherein
- said recombinant yeast strain has been modified to have an increased content and/or activity of alcohol dehydrogenase, acetaldehyde dehydrogenase and acetyl-CoA synthetase as compared to original yeast prior to modification,
- and wherein the linker peptide is SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20 or SEQ ID NO:21.
Dependencies are inferred from the claim text.