Imaging method with pulsed light
AssigneeFluoptics
InventorsAnthony Daures, Philippe Rizo
Life of this patent
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Abstract
The invention relates to a method allowing the use of the information accessible by fluorescence imaging to be optimized. For this purpose, it implements the combination of a protocol for calibration and synchronization of a pulsed light for exciting a fluorescent marker, with the operation in “rolling shutter” mode of a fluorescence camera. An appropriate correction factor allows the complete signal integrated by all of the photodiodes of the camera to be used so that no image is lost.
Claims19
A method that comprises using an apparatus to carry out imaging, wherein the apparatus comprises a sensor, a recorder, a first controller, first and second light sources, and a second controller, wherein the sensor comprises a matrix of photodiodes, wherein the first and second light sources illuminate a region-of-interest, wherein the method comprises using the recorder to record an electrical signal generated by at least one of the photodiodes, wherein the signal is used to define the brightness of a pixel on an image, using the first controller to carry out closed-loop control of the recorder for sequentially recording a signal generated by a group of photodiodes of the matrix before recording a signal generated by another group of photodiodes of the matrix, each of the groups of photodiodes comprising at least one photodiode, wherein a set that comprises the groups of photodiodes allows an image of the region-of-interest to be reconstructed, using the second controller to control activation of the first light source over a first time-period and extinction of the first light source over a second time-period, the second time-period succeeding the first time-period, for each photodiode of at least one group of photodiodes from the matrix, subtracting a second value from a first value, wherein the second value corresponds to a signal recorded during the second time-period for the photodiode and the first value corresponds to a signal recorded during the first time-period for the photodiode, determining a correction factor based on calibration measurements, and applying the correction factor to at least one value selected from the group consisting of the first value, the second value, and the result of having subtracted the second value from the first value, wherein during the calibration measurements first and second images are formed, wherein the first image is an image of a surface that is constantly reflecting light emitted by a calibration light source that emits in continuous mode throughout the sensor's exposure time, wherein the second image is an image of a surface that is constantly reflecting light emitted by the same calibration light source while the calibration light source emits light periodically over the first time-period, and wherein the period with which the calibration light source emits light periodically is the sum of the first and of the second time-period.
Dependencies are inferred from the claim text.