High temperature seed germination
AssigneeRijk Zwaan Zaadteelt En Zaadhandel B.V.
InventorsEric Roland Coppoolse, Miriam Post
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Abstract
The present invention relates to a seed comprising in its genome a modified NXS gene and/or modified regulatory sequences thereof. The modified NXS gene and/or modified regulatory sequences thereof provides the seed with the capability to germinate at a high temperature as compared to a wild type seed not having the modified NXS gene. The modification to the gene and/or its regulatory sequences may lead to the expression of the NXS gene being substantially reduced or prevented. In addition to or alternatively, the seed can have a reduced level, reduced activity or complete absence of NXS protein. The modified NXS gene may for example comprise a premature stop codon and/or encode an NXS protein that comprises one or more amino acid substitutions.
Claims13
A lettuce seed (Lactuca sativa) comprising in its genome a modified neoxanthin synthase (NXS) gene, the wild type of which is identified in SEQ ID NO:
- 1, encoding the protein of SEQ ID NO:
- 2
- wherein the modified NXS gene encodes an NXS protein, and
- wherein a proline residue is substituted with a serine residue at position 212 in the encoded protein of SEQ ID No. 2 as a result of a C>T SNP at position 3518 of SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.