Nucleosidase agent having reduced contaminant activity
AssigneesAmano Enzyme Inc., Suntory Holdings Limited
InventorsTakakiyo Obara, Keita Okuda, Tomoko Fujimura, Ikuma Mizuguchi, Tomoyuki Nakahama
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Abstract
An object of the present invention is to provide an enzyme preparation useful for reducing purine bodies, especially, in beer or beer-based beverages, and use thereof. Provided is a nucleosidase preparation having an activity ratio (U/U), which is guanine deaminase activity per nucleosidase activity, of 0.4 or less.
Claims10
A manufactured nucleosidase preparation having an activity ratio (U/U), which is guanine deaminase activity per nucleosidase activity, of 0.4 or less;
- wherein the manufactured nucleosidase preparation contains a nucleosidase having an amino acid sequence having 92% or more identity with the amino acid sequence of SEQ ID NO:
- 22 and at least one manufactured agent selected from a group comprising of an excipient, a buffer agent, a suspending agent, a stabilizer, a preservative, an antiseptic, and a saline;
- and the nucleosidase has been collected from a culture producing said nucleosidase.
A manufactured nucleosidase preparation having an activity ratio (U/U), which is guanine deaminase activity per nucleosidase activity, of 0.4 or less;
- wherein the manufactured nucleosidase preparation contains a nucleosidase having an amino acid sequence having 92% or more identity with the amino acid sequence of SEQ ID NO:
- 22 and contains at least one substitution modification relative to SEQ ID NO:
- 22;
- and the nucleosidase has been collected from a culture producing said nucleosidase.
Dependencies are inferred from the claim text.