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US 11,408,898 B2Granted
System, assay and method for partitioning proteins
AssigneeThe Brigham and Women's Hospital, Inc.
InventorsOmid Farokhzad, Morteza Mahmoudi, Claudia Corbo
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Abstract
The present disclosure provides a system comprising a communication interface and computer for assigning a label to the biomolecule fingerprint, wherein the label corresponds to a biological state. The present disclosure also provides a sensor arrays for detecting biomolecules and methods of use. In some embodiments, the sensor arrays are capable of determining a disease state in a subject.
Claims29
A method for partitioning of proteins from a biological sample, comprising:
- (a) incubating the biological sample with a plurality of different particles to form protein coronas around the plurality of different particles, wherein the biological sample comprises a fluid,
- wherein the plurality of different particles are configured to enrich low abundance proteins in the biological sample,
- wherein affinity of proteins in the biological sample for a surface of a first particle in the plurality of particles differs from a second particle in the plurality of particles such that protein coronas differently form around particles of the plurality of particles having different surface properties,
- wherein the protein coronas comprise—
- (i) a high abundance protein in the biological sample, and
- (ii) a low abundance protein present at a concentration of less than 100 ng/ml in the biological sample, and
- wherein the high abundance protein and the low abundance protein are present in different relative quantities in the protein coronas corresponding to the particles having different surface properties;
- and
- (b) collecting the protein coronas, thereby partitioning the proteins from the biological sample;
- wherein the plurality of different particles are non-covalently coupled to a substrate.
7 more elements
Dependencies are inferred from the claim text.