Lipid-free anchoring of thermophilic bacteriophage G20c portal adapter into solid-state nanopores
AssigneesNortheastern University, The University of York
InventorsMeni Wanunu, Alfred Antson, Sandra Greive, Benjamin Cressiot
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Abstract
Hybrid nanopores, comprising a protein pore supported within a solid-state membrane, which combine the robust nature of solid-state membranes with the easily tunable and precise engineering of protein nanopores. In an embodiment, a lipid-free hybrid nanopore comprises a water soluble and stable, modified portal protein of the Thermus thermophilus bacteriophage G20c, electrokinetically inserted into a larger nanopore in a solid-state membrane. The hybrid pore is stable and easy to fabricate, and exhibits low peripheral leakage, allowing sensing and discrimination among different types of biomolecules.
Claims18
A sensor comprising:
- a solid-state matrix comprising a solid-state pore opening;
- and
- a hydrophilic protein channel in a stable insertion fit within the solid-state pore opening, the hydrophilic protein channel comprising a protein nanopore channel;
- wherein a protein forming at least part of the hydrophilic protein channel comprises a modification of SEQ ID NO:
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Dependencies are inferred from the claim text.