Host cell expression of 4-hydroxyphenylacetaldehyde synthase for production of salidroside, icariside D2, and precursors
AssigneeWhitehead Institute for Biomedical Research
InventorsMichael Torrens-Spence, Jing-Ke Weng
Life of this patent
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Abstract
Transgenic host cells, vectors useful for making transgenic host cells, and kits useful for making transgenic host cells are described. Also described are transgenic plants. In some embodiments, transgenic host cells express a 4-hydroxyphenylacetaldehyde synthase (4HPAAS). In some embodiments, transgenic host cells express a tyrosol:UDP-glucose 8-O-glucosyltransferase (T8GT). The transgenic host cells are useful for biosynthesis of one or more of salidroside, icariside D2, tyrosol, and 4-hydroxypenylacetaldehyde.
Claims20
A host cell comprising a transgene encoding a heterologous 4-hydroxyphenylacetaldehyde synthase (4HPAAS) operably linked to a promoter, wherein the heterologous 4HPAAS comprises an amino acid sequence having at least 95% amino acid sequence identity to SEQ ID NO:
- 2, and wherein the amino acid sequence of the heterologous 4HPAAS comprises:
- a) an amino acid residue selected from the group consisting of L, I, M and V at a position corresponding to the F residue at position 343 in SEQ ID NO:
- 2;
- b) an amino acid residue selected from the group consisting of N and D at a position corresponding to the H residue at position 198 in SEQ ID NO:
- 2;
- or
- c) a combination thereof.
A host cell comprising a transgene encoding a heterologous 4-hydroxyphenylacetaldehyde synthase (4HPAAS) operably linked to a promoter, wherein the heterologous 4HPAAS comprises an amino acid sequence having at least 95% amino acid sequence identity to SEQ ID NO:
- 2, and wherein the amino acid sequence of the heterologous 4HPAAS comprises an amino acid residue selected from the group consisting of F, L, I, M and V at a position corresponding to the F residue at position 343 in SEQ ID NO:
- 2, and an amino acid residue selected from the group consisting of N and D at a position corresponding to the H residue at position 198 in SEQ ID NO:
- 2.
A method of making 4-hydroxyphenylacetaldehyde (4-HPAA) in a host cell, the method comprising expressing in the host cell a transgene that encodes a heterologous 4-hydroxyphenylacetaldehyde synthase (4HPAAS) operably linked to a promoter, wherein the heterologous 4HPAAS comprises an amino acid sequence having at least 95% amino acid sequence identity to SEQ ID NO:
- 2, and wherein the amino acid sequence of the heterologous 4HPAAS comprises:
- a) an amino acid residue selected from the group consisting of L, I, M and V at a position corresponding to the F residue at position 343 in SEQ ID NO:
- 2;
- b) an amino acid residue selected from the group consisting of N and D at a position corresponding to the H residue at position 198 in SEQ ID NO:
- 2;
- or
- c) a combination thereof.
A method of making 4-hydroxyphenylacetaldehyde (4-HPAA) in a host cell, the method comprising expressing in the host cell a transgene that encodes a heterologous 4-hydroxyphenylacetaldehyde synthase (4HPAAS) operably linked to a promoter, wherein the heterologous 4HPAAS comprises an amino acid sequence having at least 95% amino acid sequence identity to SEQ ID NO:
- 2, and wherein the amino acid sequence of the heterologous 4HPAAS comprises an amino acid residue selected from the group consisting of F, L, I, M and V at a position corresponding to the F residue at position 343 in SEQ ID NO:
- 2, and an amino acid residue selected from the group consisting of N and D at a position corresponding to the H residue at position 198 in SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.