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AssigneesIndian Oil Corporation Limited, Department of Biotechnology
InventorsMukund Adsul, Simranjeet Kaur Sandhu, Reeta Rani Singhania, Jitendra Kumar Saini, Anshu Shankar Mathur, Ravi Prakash Gupta, Deepak Kumar Tuli, Suresh Kumar Puri, Sankara Sri Venkata Ramakumar
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The present invention relates to a mutant fungal strain of Penicillium funiculosum ‘MRJ-16’ characterized by the ability to produce high titer of enzyme mixture comprising FPase, CMCase, Cellobiase, β-glucosidase, endoglucanase, α-L arabinofuranosidase, β-xylosidase, xylanase, pectinase, cellobiohydrase and oxidases and produce enzymes in the presence of a catabolite repressor molecule like glucose and/or xylose. The titer of enzyme mixture produced using mutant fungal strain MRJ-16 is at least two fold higher than naive Penicillium funiculosum strain NCIM 1228, when used in a fermentation process. The mutant strain ‘MRJ-16’ with high hydrolytic activity and catabolite derepressed character is having application in the method of degrading or saccharifying biomass to produce valuable products for example-bioethanol.
A mutant fungal strain, wherein the mutant fungal strain is Penicillium funiculosum MRJ 16, having Microbial Type Culture Collection (MTCC) Accession Number 25142, wherein the mutant fungal strain is characterized by the ability to produce at least two fold higher titer of an enzyme mixture comprising filter paper enzyme, carboxymethyl cellulase, Cellobiase, β-glucosidase, endoglucanase, α-L arabinofuranosidase, β-xylosidase, xylanase, pectinase, cellobiohydrase and oxidases as compared to a native Penicillium funiculosum NCIM 1228 strain;
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