P aeruginosa PcrV-linked antigen vaccines
AssigneeGlaxosmithkline Biologicals SA
InventorsChristiane Marie-Paule Simone Jeanne Feron, Stefan Jochen Kemmler, Michael Thomas Kowarik, Julien Laurent Quebatte
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Abstract
The present invention discloses a conjugate comprising an antigen (for example a saccharide antigen) covalently linked to a Pseudomonas aeruginosa PcrV carrier protein comprising an amino acid sequence which is at least 80% identical to the sequence of SEQ ID NO:1-4, wherein the antigen is linked (either directly or through a linker) to an amino acid residue of the P. aeruginosa PcrV carrier protein. The invention also discloses Pseudomonas aeruginosa PcrV proteins that contain glycosylation site consensus sequences.
Claims6
A conjugate comprising or consisting of an antigen covalently linked to a Pseudomonas aeruginosa PcrV carrier protein comprising an amino acid sequence which is at least 90% identical to the sequence of SEQ ID NO:
- 4, wherein the antigen is linked (either directly or through a linker) to an asparagine amino acid residue of the P. aeruginosa PcrV carrier protein wherein the asparagine residue is part of a D/E-X-N-X-S/T consensus sequence introduced into the amino acid sequence which is at least 90% identical to the sequence of SEQ ID NO:
- 4, wherein X is any amino acid apart from proline, wherein the asparagine residue is situated at a position equivalent to between amino acids 1-50 of SEQ ID NO:4;
- wherein the PerV carrier protein elicits a neutralizing immune response against PcrV:
- wherein a peptide comprising the D/E-X-N-X-S/T consensus sequence is introduced into the amino acid sequence by the removal of a PcrV peptide sequence and its replacement with the peptide comprising the D/E-X-N-X-S/T consensus sequence, wherein the PcrV peptide sequence contains 1-7 amino acids, wherein the PcrV peptide sequences contains one amino acid.
Dependencies are inferred from the claim text.