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US 11,401,524 B2Granted
Methods and compositions for genome editing via haploid induction
AssigneeMonsanto Technology LLC
InventorsCharles L. Armstrong, Edward J. Cargill, Fenggao Dong, Jonathan C. Lamb, Huachun W. Larue, Richard J. Lawrence, Thomas S. Ream
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Abstract
Methods and compositions for improved plant breeding using gene editing and haploid induction are provided.
Claims16
A method of editing maize genomic DNA, comprising:
- a) introducing into the genome of a maize haploid inducer line a nucleic acid sequence encoding at least one Genome Editing Component (GEC) to produce a first maize plant, wherein the genome of the maize haploid inducer comprises a supernumerary chromosome, and wherein the at least one GEC is introduced into the supernumerary chromosome;
- b) providing a second maize plant, wherein the second maize plant comprises the maize plant genomic DNA which is to be edited;
- c) crossing the second maize plant with the first maize plant;
- and
- d) selecting at least one haploid progeny produced by the cross of step (c) wherein the haploid progeny comprises the genome of the second maize plant and the supernumerary chromosome from said first maize plant but otherwise lacks the genome of the first maize plant, and wherein the genome of the haploid progeny comprises at least one targeted genome modification.
2 more elements
A method of editing maize genomic DNA, comprising:
- a) introducing into the genome of a maize haploid inducer line a nucleic acid sequence encoding at least one Genome Editing Component (GEC) to produce a first maize plant wherein the genome of the maize haploid inducer comprises a supernumerary chromosome, and wherein the at least one GEC is introduced into the supernumerary chromosome;
- b) providing a second maize plant, wherein the second maize plant comprises the maize plant genomic DNA which is to be edited;
- c) crossing the second maize plant with the first maize plant;
- d) selecting at least one haploid progeny produced by the cross of step (c) wherein the haploid progeny comprises the genome of the second maize plant and the supernumerary chromosome from said first maize plant but otherwise lacks the genome of the first maize plant;
- e) crossing the progeny from step (d) with itself or a third maize plant;
- and
- f) selecting offspring from the progeny of step (e) wherein the progeny lacks the supernumerary chromosome from said first plant and comprises the at least one targeted genome modification.
4 more elements
Dependencies are inferred from the claim text.