Sequential co-culturing method for producing a vitamin- and protein-rich food product

A method for producing a vitamin- and protein-rich product, comprising the steps:

• a) Cultivating in a vessel, at least one species of the division of Basidiomycota submerged in a nutrient medium comprising at least one carbohydrate-containing agricultural waste or food waste to obtain a first cultivation product;
• b) Adding at least one vitamin B12-producing species of the genus Propionibacterium and/or of the genus Lactobacillus to the first cultivation product, wherein the at least one vitamin B12-producing species is selected from the group consisting of Propionibacterium freudenreichii sups. freudenreichii, Propionibacterium freudenreichii sups. shermanii, Lactobacillus reuteri, and combination thereof;
• and
• c) Cultivating the at least one species of the genus Propionibacterium and/or at least one species of the genus Lactobacillus in the first cultivation product to obtain a second cultivation product,
• wherein the at least one species of the division of Basidiomycota is selected from the group consisting of Agrocybe aegerita, Pleurotus roseus, Lentinula edodes, Laetiporus sulphureus, Pleurotus sapidus, Stropharia rugosoannulata, Wolfiporia cocos, and mixtures thereof;
• wherein the second cultivation product is the vitamin- and protein-rich product.