Method for determining molecular probe

A method for determining a molecular probe that captures a target compound, the method comprising:

• (i) electrophoresing a plurality of kinds of candidate molecules on a gel so as to obtain a plurality of bands of the candidate molecules on the gel (a first electrophoresis);
• (ii) making the target compound contact the plurality of bands obtained by the first electrophoresis so as to obtain a plurality of kinds of mixtures of the candidate molecules after the first electrophoresis and the target compound on the bands obtained by the first electrophoresis, and electrophoresing the plurality of kinds of mixtures in a direction orthogonal to a direction of the first electrophoresis (a second electrophoresis), the second electrophoresis obtaining at least one band on the gel containing one of the candidate molecules, which is broader than at least one band of the plurality of bands obtained by the first electrophoresis in the direction of the second electrophoresis on the gel;
• (iii) determining the candidate molecules contained in the at least one band of the bands of the second electrophoresis as the molecular probe that captures the target compound;
• and
• (iv) extracting the candidate molecules determined as the molecular probe that captures the target compound, wherein
• an electrophoresis method used for the first electrophoresis and the second electrophoresis is the same.

A method for determining a molecular probe that captures a target compound, the method comprising:

• (i) making a plurality of kinds of candidate molecules into contact with a target molecule and electrophoresing an obtained mixture on a gel so as to obtain a plurality of bands of the candidate molecules on the gel (a first electrophoresis);
• (ii) making the target compound dissociate the plurality of bands obtained by the first electrophoresis so as to obtain a plurality of kinds of mixtures of the candidate molecules after the first electrophoresis and the target compound on the bands obtained by the first electrophoresis, and electrophoresing the plurality of kinds of mixtures in a direction orthogonal to the direction of the first electrophoresis (a second electrophoresis), the second electrophoresis obtaining a plurality of bands of the candidate molecules on the gel, into which at least one band of the plurality of bands obtained by the first electrophoresis is separated by the second electrophoresis in the direction of the first electrophoresis on the gel;
• (iii) determining the candidate molecules contained in the at least one band of the second electrophoresis as the molecular probe that captures the target compound;
• and
• (iv) extracting the candidate molecules determined as the molecular probe that captures the target compound,
• wherein the first electrophoresis and the second electrophoresis are performed by the same electrophoresis method.

A method for determining a molecular probe that captures a target compound,

• the method comprising:
• (i) making a plurality of kinds of candidate molecules into contact with a target molecule and electrophoresing an obtained mixture on a gel so as to obtain a plurality of bands of the candidate molecules on the gel (a first electrophoresis);
• (ii) making the target compound dissociate the plurality of bands obtained by the first electrophoresis so as to obtain a plurality of kinds of mixtures of the candidate molecules after the first electrophoresis and the target compound on the bands obtained by the first electrophoresis, and electrophoresing the plurality of kinds of mixtures in a direction orthogonal to the direction of the first electrophoresis (a second electrophoresis), the second electrophoresis obtaining at least one band on the gel containing one of the candidate molecules, which is broader than at least one band of the plurality of bands obtained by the first electrophoresis in the direction of the first electrophoresis on the gel;
• (iii) determining the candidate molecules contained in the at least one band of the second electrophoresis as the molecular probe that captures the target compound;
• and
• (iv) extracting the candidate molecules determined as the probe that captures the target compound,
• wherein the first electrophoresis and the second electrophoresis are performed by the same electrophoresis method.