Back to results
US 11,390,891 B2Granted
Recombinant host cells and methods for the production of D-lactic acid
AssigneeLygos, Inc.
InventorsJeffrey A. Dietrich, Mario Ouellet, Andrew Jonathan Conley
Life of this patent
Estimating the term…
Abstract
Methods and materials related to producing D-lactic acid are disclosed. Specifically, isolated synthetic or natural nucleic acids, synthetic or natural polypeptides, host cells, and methods and materials for producing D-lactic acid by direct fermentation from carbon sources are disclosed, along with methods of preparing D-lactic acid polymers.
Claims15
A recombinant cell, comprising:
- a heterologous nucleic acid encoding a D-lactate dehydrogenase selected from SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 17, SEQ ID NO:
- 18, SEQ ID NO:
- 19, SEQ ID NO:
- 20, SEQ ID NO:
- 21, SEQ ID NO:
- 22 or SEQ ID NO:
- 23, wherein:
- the D-lactate dehydrogenase nucleic acid comprises the residues GXGXXG, wherein X refers to any amino acid;
- a negatively charged amino acid located 18-20 residues downstream from the GXGXXG sequence is mutated to a neutral amino acid;
- and
- wherein the heterologous nucleic acid is expressed in sufficient amount to produce D-lactic acid.
15 more elements
A recombinant Pichia kudriavzevii cell, comprising a heterologous nucleic acid encoding D-lactate dehydrogenase selected from SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 18, SEQ ID NO:
- 19, SEQ ID NO:
- 20, SEQ ID NO:
- 21, SEQ ID NO:
- 22, and SEQ ID NO:
- 23, wherein:
- the D-lactate dehydrogenase nucleic acid comprises the residues GXGXXG, wherein X refers to any amino acid;
- and
- a negatively charged amino acid located 18-20 residues downstream from the GXGXXG sequence is mutated to a neutral amino acid.
10 more elements
Dependencies are inferred from the claim text.