Method for manufacturing 1,3-propanediol
AssigneesGreen Earth Institute Co., Ltd., Natural Beauty, Limited
InventorsKeisuke Yamamoto, Atsunari Tsuchisaka, Shuhei Nakane, Toru Nakayashiki
Life of this patent
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Abstract
A method for manufacturing 1,3-propanediol includes culturing, in the presence of a saccharide and formaldehyde to produce 1,3-propanediol, a microorganism having the following genes: (a) a first gene encoding an enzyme that catalyzes an aldol reaction between pyruvic acid and aldehydes; (b) a second gene encoding an enzyme that catalyzes a decarboxylation reaction of α-keto acids; and (c) a third gene encoding an enzyme that catalyzes a reduction reaction of aldehydes, is provided.
Claims15
A method for manufacturing 1,3-propanediol, comprising culturing, in the presence of a saccharide and formaldehyde to produce 1,3-propanediol, a microorganism including the following genes:
- (a) a first gene encoding an enzyme having EC numbers selected from the group consisting of EC NO:
- 4.1.3.39, EC NO:
- 4.1.2.14, EC NO:
- 4.1.2.55, EC NO:
- 4.1.3.16, EC NO:
- 4.1.3.42, EC NO:
- 4.1.2.28, EC NO:
- 4.1.2.51, EC NO:
- 4.1.2.23, EC NO:
- 4.1.2.34, EC NO:
- 4.1.2.52, EC NO:
- 4.1.2.20, EC NO:
- 4.1.2.53, EC NO:
- 4.1.3.17 and EC NO:
- 4.1.3.43, that catalyzes an aldol reaction between pyruvic acid and formaldehyde;
- (b) a second gene encoding an enzyme having EC numbers selected from the group consisting of EEC NO:
- 4.1.1.7, EC NO:
- 4.1.1.1 and EC NO:
- 4.1.1.74 that catalyzes a decarboxylation reaction of 4-hydroxy-2-oxobutyric acid;
- and
- (c) a third gene encoding an enzyme having EC NO:
- 1.1.1.202 that catalyzes a reduction reaction of 3-hydroxypropionaldehyde.
Dependencies are inferred from the claim text.