Compositions and methods for succinate production
AssigneeCargill, Incorporated
InventorsKenneth R. Finley, Jeanette M. Huryta, Beth M. Mastel, Thomas W. McMullin, Gregory M. Poynter, Brian J. Rush, Arlene M. Fosmer, Vernon L. McIntosh, Jr., Keith M. Brady, Kevin T. Watts
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Abstract
The present application provides genetically modified yeast cell comprising an active succinate fermentation pathway, as well as methods of using these cells to produce succinate.
Claims20
A method of producing succinate comprising culturing genetically modified yeast cells in the presence of at least one carbon source and isolating succinate from the culture, wherein the genetically modified yeast cells are from the Pichia fermentans/Issatchenkia orientalis clade and have an active succinate fermentation pathway from phosphoenolpyruvate or pyruvate to succinate, wherein the active succinate fermentation pathway comprises the reactions:
- (a) pyruvate to oxaloacetate;
- (b) oxaloacetate to malate;
- (c) malate to fumarate;
- and
- (d) fumarate to succinate,
- wherein the cells comprise an exogenous succinate exporter gene that catalyzes export of succinate from inside the cell to the extracellular environment.
A method of producing succinate comprising culturing genetically modified yeast cells in the presence of at least one carbon source and isolating succinate from the culture, wherein the genetically modified yeast cells are from the species Issatchenkia orientalis, Pichia kudriavzevii, Candida kruzei, or Candida lambica and have an active succinate fermentation pathway from phosphoenolpyruvate or pyruvate to succinate, wherein the active succinate fermentation pathway comprises the reactions:
- (a) pyruvate to oxaloacetate;
- (b) oxaloacetate to malate;
- (c) malate to fumarate;
- and
- (d) fumarate to succinate,
- wherein the cells comprise an exogenous succinate exporter, gene that catalyzes export of succinate from inside the cell to the extracellular environment.
Dependencies are inferred from the claim text.