Santalene synthase
AssigneeIsobionics B.V.
InventorsMartinus Julius Beekwilder, Adèle Margaretha Maria Liduina Van Houwelingen, Hendrik Jan Bosch, Georg Friedrich Lentzen, Elena Melillo, Hendrik Wouter Wisselink
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Abstract
The invention is directed to a santalene synthase, to a nucleic acid encoding said santalene synthase, to an expression vector comprising said nucleic acid, to a host cell comprising said expression vector, to a method of preparing santalene, to a method of preparing santalol and to a method of preparing a santalene synthase. The invention is further directed to an antibody specific for the santalane synthase.
Claims21
A santalene synthase comprising a functional homologue of an amino acid sequence as shown in SEQ ID NO:
- 3 An isolated santalene, said homologue being a santalene synthase comprising an amino acid sequence which has a sequence identity of at least 95% with SEQ ID NO:
- 3, wherein said functional homologue does not have 100% identity with SEQ ID NO:
- 3.
An expression vector comprising a nucleic acid sequence encoding a santalene synthase comprising an amino acid sequence as shown in SEQ ID NO:
- 3 or a functional homologue thereof, said homologue being a santalene synthase comprising an amino acid sequence which has a sequence identity of at least 95% with SEQ ID NO:
- 3.
A host cell, which may be an organism per se or part of a multi-cellular organism, said host cell comprising an expression vector comprising a heterologous nucleic acid sequence encoding a santalene synthase comprising an amino acid sequence as shown in SEQ ID NO:
- 3 or a functional homologue thereof, said homologue being a santalene synthase comprising an amino acid sequence which has a sequence identity of at least 95% with SEQ ID NO:
- 3.
A method for preparing santalene in a transformed host cell, a transgenic plant or plant culture, or a mushroom or mushroom culture, comprising converting a farnesyl diphosphate to santalene in the presence of a recombinant santalene synthase comprising an amino acid sequence as shown in SEQ ID NO:
- 3 or a functional homologue thereof, said homologue being a santalene synthase comprising an amino acid sequence which has a sequence identity of at least 95% with SEQ ID NO:
- 3.
A method for preparing santalol, comprising converting farnesyl diphosphate to santalene in the presence of an isolated or recombinant santalene synthase comprising an amino acid sequence as shown in SEQ ID NO:
- 3 or a functional homologue thereof, said homologue being a santalene synthase comprising an amino acid sequence which has a sequence identity of at least 95% with SEQ ID NO:
- 3, further comprising converting the santalene into santalol.
Dependencies are inferred from the claim text.