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US 11,390,862 B2Granted
Proteinases with improved properties
AssigneeNew England Biolabs, Inc.
InventorsMinyong Chen, James C. Samuelson, Ming-Qun Xu, Aihua Zhang, Margaret Heider, Pingfang Liu
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Abstract
Provided herein is a thermolabile proteinase and methods of using the same. In some embodiments, the thermolabile proteinase may comprise an amino acid sequence that is at least 90% identical to any of SEQ ID NOs:1-11 and at least one amino acid substitution in helix 3. The thermolabile proteinase is active at a temperature in the range of 4° C.-40° C. and is inactivated by raising the temperature to above 50° C., where the proteinase is substantially inactive at 65° C.
Claims8
A method for degrading protein, comprising:
- incubating a sample comprising the protein with a thermolabile protease at a temperature that is below 50° C., wherein
- i. the thermolabile protease has an amino acid sequence is at least 90% identical to SEQ ID NO:
- 1 and
- ii. the amino acid at the position corresponding to position 236 in SEQ ID NO:
- 1 in the protease is an amino acid other than tyrosine but not proline.
2 more elements
Dependencies are inferred from the claim text.