Compositions and methods of preparing airway cells
AssigneeYale University
InventorsMahboobe Ghaedi, Laura Niklason
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Abstract
The present invention provides compositions and methods of preparing airway cells. In one aspect, an epithelial airway cell derived from an induced pluripotent stem (iPS) cell characterized by expression of airway cell surface markers and an ability to proliferate is described. In another aspect, methods of differentiating an iPS into an epithelial airway cell is provided. Engineered lungs, methods of making such engineered lungs comprising the epithelial airway cells and treating respiratory disorders are also disclosed.
Claims7
An airway epithelial cell derived from an induced pluripotent stem (iPS) cell characterized by:
- expression of airway cell surface markers, wherein the airway cell surface markers comprise Clara cell secretory protein (CCSP), cytokeratin 5 (KRT5), and FOXJ1;
- and
- an ability to proliferate in culture for at least about 30 days without loss of the airway cell surface markers;
- wherein the iPS cell is cultured in the absence of serum to induce differentiation into a definitive endoderm (DE) cell;
- wherein the DE cell is cultured in the presence of serum to induce differentiation into an anterior foregut endoderm (AFE) cell;
- and
- wherein the AFE cell is cultured in the presence of serum, a cytokine cocktail that inhibits WNT pathway, and at least 0.5 μM retinoic acid to induce differentiation of the AFE cell into the airway epithelial cell.
Dependencies are inferred from the claim text.