Compositions comprising a polymeric dye and a cyclodextrin and uses thereof

A composition comprising a polymeric dye and a cyclodextrin, wherein the polymeric dye is a compound having the following structure (I):

• or a stereoisomer, salt or tautomer thereof, wherein:
• M is, at each occurrence, independently a bivalent pyrene, 6-FAM, dimethylaminostilbene, quinacridone, fluorophenyl-dimethyl-BODIPY, his-fluorophenyl-BODIPY, acridine, terrylene, sexiphenyl, porphyrin, benzopyrene, (fluorophenyl-dimethyl-difluorobora-diaza-indacene)phenyl, (bis-fluorophenyl-difluorobora-diaza-indacene)phenyl, quaterphenyl, bi-benzothiazole, ter-benzothiazole, bi-naphthyl, bi-anthracyl, squaraine, squarylium, 9,10-ethynylanthracene, ter-naphthyl moiety, p-terphenyl, perylene, azobenzene, phenazine, phenanthroline, acridine, thioxanthrene, chrysene, rubrene, coronene, cyanine, perylene imide, perylene monoimide, perylene amide, coumarin dye, resorufin dye, dipyrrometheneboron difluoride dye, ruthenium bipyridyl dye, energy transfer dye, thiazole orange dye, polymethine, or N-aryl-1,8-naphthalimide dye;
• L1 is at each occurrence, independently an optional alkylene or heteroalkylene linker;
• L3 is, at each occurrence, independently an optional alkylene linker;
• L4 is, at each occurrence, independently an alkylene oxide linker of greater than three atoms in length;
• R2 and R3 are each independently H, OH, SH, alkyl, alkoxy, alkylether, heteroalkyl, —OP(═Ra)(Rb)Rc, Q, or a protected form thereof, or L′;
• Ra is O or S;
• Rb is OH, SH, O−, S−, ORd or SRd;
• Rc is OH, SH, O−, S−, ORd, OL′, SRd, alkyl, alkoxy, heteroalkyl, heteroalkoxy, alkylether, alkoxyalkylether, phosphate, thiophosphate, phosphoalkyl, thiophosphoalkyl, phosphoalkylether or thiophosphoalkylether;
• Rd is a counter ion;
• Q is, at each occurrence, independently a moiety comprising sulfhydryl, disulfide, N-succinimide ester, imidoester, polyfluorophenyl ester, isothiocyanate, azide, alkyne, alkene, diene, dienophile, acid halide, sulfonyl halide, phosphine, α-haloamide, biotin, amino or maleimide functional group;
• L′ is, at each occurrence, independently a linker comprising a covalent bond to Q, a linker comprising a covalent bond to a targeting moiety, a linker comprising a covalent bond to an analyte molecule, a linker comprising a covalent bond to a solid support, a linker comprising a covalent bond to a solid support residue, a linker comprising a covalent bond to a nucleoside or a linker comprising a covalent bond to a further compound of structure (I);
• m is, at each occurrence, independently an integer of zero or greater, provided that at least one occurrence of m is an integer of one or greater;
• and
• n is an integer of one or greater.