Amino acid-specific binder and selectively identifying an amino acid

A process for selectively identifying an N-terminal amino acid, the process comprising:

• providing an analyte comprising a protein, a peptide, an amino acid, or a combination comprising at least one of foregoing;
• contacting a C-terminal end of the analyte with an anchor;
• anchoring the C-terminal end to the anchor to form an anchored analyte;
• contacting an N-terminal amino acid of the anchored analyte with a binder complex, the binder complex comprising:
• an amino acid-specific binder;
• and
• a taggant attached to the amino acid-specific binder;
• selectively binding the amino acid-specific binder of the binder complex to the N-terminal amino acid of the anchored analyte when the N-terminal amino acid comprises a binding amino acid to form a tagged complex;
• subjecting the taggant of the tagged complex to a stimulus;
• producing, by the taggant of the tagged complex, a taggant signal in response to the stimulus;
• detecting the taggant signal;
• and
• identifying the N-terminal amino acid based on the taggant signal, wherein the amino acid-specific binder binds selectively to the binding amino acid, and
• the amino acid-specific binder comprises the amino acid sequence comprising:
• a first amino acid sequence comprising X1-C-P-S-X2-V-X3-R-X4-T-X5-C-E-X6-E-X7-G-K-X8;
• a second amino acid sequence comprising X1-C-S-W-X2-V-X3-R-X4-T-X5-C-E-X6-E-X7-G-K-X8;
• a third amino acid sequence comprising X1-P-M-S-X2-V-X3-R-X4-T-X5-C-E-X6-E-X7-G-K-X8;
• a fourth amino acid sequence comprising X1-S-G-R-X2-V-X3-R-X4-T-X5-C-E-X6-E-X7-G-K-X8;
• a fifth amino acid sequence comprising X1-P-M-P-X2-V-X3-R-X4-T-X5-C-E-X6-E-X7-G-K-X8;
• a sixth amino acid sequence comprising X1-P-R-E-X2-V-X3-R-X4-T-X5-S-E-X6-E-X7-G-K-X8;
• a seventh amino acid sequence comprising X1-P-R-E-X2-E-X3-N-X4-Q-X5-C-T-X6-Q-X7-A-R-X8;
• or an eighth amino acid sequence comprising X1-P-M-S-X2-E-X3-N-X4-Q-X5-S-T-X6-Q-X7-A-R-X8;
• wherein:
• X1 comprises an amino acid sequence comprising
• SDSPVDLKPKPKVKPKLERPKLYKVMLLNDDYT (Sequence ID No. 20);
• X2 comprises an amino acid sequence comprising FVT;
• X3 comprises an amino acid sequence comprising VLKAVF (Sequence ID No. 21);
• X4 comprises an amino acid sequence comprising MSED (Sequence ID No. 22);
• X5 comprises an amino acid sequence comprising GRRVMMTAHRFGSAVVVV (Sequence ID No. 23);
• X6 comprises an amino acid sequence comprising RDIAETKAK (Sequence ID No. 24);
• X7 comprises an amino acid sequence comprising ATDL (Sequence ID No. 25);
• and
• X8 comprises an amino acid sequence comprising EAGFPLMFTTEPEE (Sequence ID No. 26),
• such that a total percentage amount of substitutions and deletions to X1, X2, X3, X4, X5, X6, X7, and X8 is from 0% to less than 30%, exclusive of SDSPVDLKPKPKVKPKLERPKLYKVMLLNDDYTPMEFVTVVLKAVFRMSEDTGRRVM MTAHRFGSAVVVVCERDIAETKAKEATDLGKEAGFPLMFTTEPEE (Sequence ID No. 27), or
• wherein the amino acid-specific binder comprises the amino acid sequence comprising NLEKIKKLRNVIKEIKKDNIKEADEHEKKEREKETSAWKVILYNDDIHKFSYVTDVIVKVV GQISKAKAHTITVEAHSTGQALILSTQKSKAEKYCQELQQNGLTVSIIHESQLKDKQKK (Sequence ID No. 10).

A process for selectively isolating an analyte, the process comprising:

• contacting an amino acid-specific binder with an analyte comprising a protein, a peptide, an amino acid, or a combination comprising at least one of foregoing;
• selectively binding the amino acid-specific binder to the N-terminal amino acid of the analyte when the N-terminal amino acid comprises a binding amino acid to form an isolation complex;
• separating the isolation complex from a fluid in which the isolation complex is disposed to selectively isolating the analyte,
• wherein the amino acid-specific binder binds selectively to the binding amino acid, and the amino acid-specific binder comprises the amino acid sequence comprising:
• a first amino acid sequence comprising X1-C-P-S-X2-V-X3-R-X4-T-X5-C-E-X6-E-X7-G-K-X8;
• a second amino acid sequence comprising X1-C-S-W-X2-V-X3-R-X4-T-X5-C-E-X6-E-X7-G-K-X8;
• a third amino acid sequence comprising X1-P-M-S-X2-V-X3-R-X4-T-X5-C-E-X6-E-X7-G-K-X8;
• a fourth amino acid sequence comprising X1-S-G-R-X2-V-X3-R-X4-T-X5-C-E-X6-E-X7-G-K-X8;
• a fifth amino acid sequence comprising X1-P-M-P-X2-V-X3-R-X4-T-X5-C-E-X6-E-X7-G-K-X8;
• a sixth amino acid sequence comprising X1-P-R-E-X2-V-X3-R-X4-T-X5-S-E-X6-E-X7-G-K-X8;
• a seventh amino acid sequence comprising X1-P-R-E-X2-E-X3-N-X4-Q-X5-C-T-X6-Q-X7-A-R-X8;
• or an eighth amino acid sequence comprising X1-P-M-S-X2-E-X3-N-X4-Q-X5-S-T-X6-Q-X7-A-R-X8;
• wherein:
• X1 comprises an amino acid sequence comprising
• SDSPVDLKPKPKVKPKLERPKLYKVMLLNDDYT (Sequence ID No. 20);
• X2 comprises an amino acid sequence comprising FVT;
• X3 comprises an amino acid sequence comprising VLKAVF (Sequence ID No. 21);
• X4 comprises an amino acid sequence comprising MSED (Sequence ID No. 22);
• X5 comprises an amino acid sequence comprising GRRVMMTAHRFGSAVVVV (Sequence ID No. 23);
• X6 comprises an amino acid sequence comprising RDIAETKAK (Sequence ID No. 24);
• X7 comprises an amino acid sequence comprising ATDL (Sequence ID No. 25);
• and
• X8 comprises an amino acid sequence comprising EAGFPLMFTTEPEE (Sequence ID No. 26),
• such that a total percentage amount of substitutions and deletions to X1, X2, X3, X4, X5, X6, X7, and X8 is from 0% to less than 30%, exclusive of SDSPVDLKPKPKVKPKLERPKLYKVMLLNDDYTPMEFVTVVLKAVFRMSEDTGRRVM MTAHRFGSAVVVVCERDIAETKAKEATDLGKEAGFPLMFTTEPEE (Sequence ID No. 27), or
• wherein the amino acid-specific binder comprises the amino acid sequence comprising NLEKIKKLRNVIKEIKKDNIKEADEHEKKEREKETSAWKVILYNDDIHKFSYVTDVIVKVV GQISKAKAHTITVEAHSTGQALILSTQKSKAEKYCQELQQNGLTVSIIHESQLKDKQKK (Sequence ID No. 10).