Bacterial consortium for reducing perchlorate and/or nitrate and the process thereof
AssigneeCouncil of Scientific and Industrial Research
InventorsKrishnakumar Bhaskaran, Anupama Vijaya Nadaraja
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Abstract
The present invention discloses a novel microbial process for decontaminating (per)chlorate and/or nitrate containing matrices. A heterotrophic mixed microbial culture expressing the functional genes responsible for (per) chlorate and nitrate reduction is the major component of the process. The present process can be a better substitute for existing processes for decontaminating perchlorate contaminated propellant wastewater, ion exchange resin/regenerate solutions, etc. The consortium consists or comprises of Halomonas sp. NIIST-PRB-02 (MTCC No. 5911), Bacillus sp. NIIST-PRB-03 (MTCC No. 5912) and Serratia marcescens strain NIIST5 (MTCC No. 5821).
Claims10
A bioprocess for detoxifying (per)chlorate and/or nitrate containing matrices using a bacterial consortium, said process comprising:
- (a) maintaining a bacterial consortium and providing a substrate and nutrient broth and conditions for sustaining microbial activity of the consortium;
- and
- (b) contacting a (per)chlorate and/or nitrate contaminated matrix with the bacterial consortium at a redox potential below −200 mV, neutral pH and 30±5° C. for a period ranging between 3 to 36 h to obtain a detoxified matrix, wherein the nitrate concentration before contacting is ≥5 g/L;
- wherein said bacterial consortium consists of bacteria having Accession No. MTCC No. 5911, MTCC No. 5912, and MTCC No. 5821.
Dependencies are inferred from the claim text.