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US 11,385,240 B2Granted
Reagents and methods for detecting protein lysine 3-hydroxybutyrylation
AssigneePtm Bio LLC
InventorsYingming Zhao, Zhongyu Xie
Life of this patent
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Abstract
The invention provides an isolated peptide comprising a lysine 3-hydroxybutyrylation site, a lysine 3-hydroxybutyrylation specific affinity reagent that specifically binds to the peptide, and a method for detecting protein lysine 3-hydroxybutyrylation in a sample using the reagent.
Claims6
A method for detecting a 3-hydroxybutyrylated lysine in a protein or a fragment thereof, comprising:
- (a) immunizing a host with an isolated peptide comprising a 3-hydroxybutyrylated lysine, whereby an affinity reagent that binds specifically to the peptide is produced,
- (b) contacting the protein or a fragment thereof with the affinity reagent, whereby the affinity reagent and the protein or a fragment thereof forms a binding complex, and
- (c) detecting the binding complex, wherein the presence of the binding complex indicates the presence of a 3-hydroxybutyrylated lysine in the protein or a fragment thereof.
A method for detecting a 3-hydroxybutyrylated lysine in a protein or a fragment thereof, comprising:
- (a) screening a peptide library using an isolated peptide comprising a 3-hydroxybutyrylated lysine, whereby an affinity reagent that binds specifically to the peptide is produced,
- (b) contacting the protein or a fragment thereof with the affinity reagent, whereby the affinity reagent and the protein or a fragment thereof forms a binding complex, and
- (c) detecting the binding complex, wherein the presence of the binding complex indicates the presence of a 3-hydroxybutyrylated lysine in the protein or a fragment thereof.
Dependencies are inferred from the claim text.