Chemigenetic voltage indicators
AssigneeHoward Hughes Medical Institute
InventorsEric R. Schreiter, Luke D. Lavis, Ahmed Abdelfattah
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Abstract
Provided herein are a voltage indicator and a method of measuring voltage. The voltage indicator includes a membrane-localized voltage-sensitive protein coupled to a capture protein. The method of measuring voltage includes administering a voltage indicator including a membrane-localized voltage-sensitive protein coupled to a capture protein, and determining changes in fluorescence of a small-molecule fluorescent dye captured by the capture protein.
Claims20
A voltage indicator comprising a membrane-localized voltage sensitive protein coupled to a capture protein, wherein the capture protein is covalently or noncovalently labeled with one or more cell permeable fluorescent dyes.
A method of measuring voltage, the method comprising administering one or more cell permeable fluorescent dyes and a voltage indicator comprising a membrane-localized voltage sensitive protein coupled to a capture protein and determining measuring changes in fluorescence when the one or more cell permeable fluorescent dye are captured by the covalent-capture protein of the voltage.
Dependencies are inferred from the claim text.