Metabolically engineered organisms for the production of added value bio-products
AssigneeInbiose N.V.
InventorsJo Maertens, Joeri Beauprez, Marjan De Mey
Life of this patent
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Abstract
The present invention relates to genetically engineered organisms, especially microorganisms such as bacteria and yeasts, for the production of added value bio-products such as specialty saccharide, activated saccharide, nucleoside, glycoside, glycolipid or glycoprotein. More specifically, the present invention relates to host cells that are metabolically engineered so that they can produce said valuable specialty products in large quantities and at a high rate by bypassing classical technical problems that occur in biocatalytical or fermentative production processes.
Claims19
A metabolically engineered bacterium or yeast for the production of glucose-1-phosphate, UDP-glucose, or glucose-6-phosphate, characterized in that said bacterium or yeast
- has been genetically modified by introducing a heterologous gene encoding a sucrose phosphorylase capable of splitting sucrose into glucose-1-phosphate and fructose, and
- a) comprises a phosphoglucomutase and has been further genetically modified to prevent loss of glucose-1-phosphate via glycolysis due to genetic disruption of an endogenous gene encoding a glucose-1-phosphatase, a glucose-1-phosphate adenylyltransferase, or a combination thereof;
- or
- b) comprises a UDP-glucose pyrophosphorylase and has been further genetically modified to prevent loss of glucose-1-phosphate via glycolysis due to genetic disruption of an endogenous gene encoding a phosphoglucomutase, a glucose-1-phosphatase, a glucose-1-phosphate adenylyltransferase, or a combination thereof.
A metabolically engineered bacterium or yeast for the production of UDP-glucose, characterized in that said bacterium or yeast cell:
- (a) has been genetically modified by introducing a heterologous gene encoding a sucrose phosphorylase capable of splitting sucrose into glucose-1-phosphate and fructose;
- (b) has been further genetically modified to prevent loss of glucose-1-phosphate via glycolysis due to genetic disruption of an endogenous gene encoding a phosphoglucomutase, a glucose-1-phosphatase, a glucose-1-phosphate adenylyltransferase, or a combination thereof;
- and
- (c) comprises a UDP-glucose pyrophosphorylase to catalyze conversion of glucose-1-phosphate and UTP to UDP-glucose.
A metabolically engineered bacterium or yeast for the production of glucose-6-phosphate, characterized in that said bacterium or yeast:
- a) has been genetically modified by introducing a heterologous gene encoding a sucrose phosphorylase capable of splitting sucrose into glucose-1-phosphate and fructose;
- and
- b) has been further genetically modified to prevent loss of glucose-1-phosphate via glycolysis due to genetic disruption of an endogenous gene encoding a glucose-1-phosphatase, a glucose-1-phosphate adenylyltransferase, or a combination thereof;
- and
- (c) comprises a phosphoglucomutase to catalyze conversion of glucose-1-phosphate to glucose-6-phosphate.
A metabolically engineered bacterium or yeast for the production of glucose-1-phosphate, characterized in that said bacterium or yeast:
- a) has been genetically modified by introducing a heterologous gene encoding a sucrose phosphorylase capable of splitting sucrose into glucose-1-phosphate and fructose;
- and
- b) has been further genetically modified to prevent loss of glucose-1-phosphate via glycolysis due to genetic disruption of an endogenous gene encoding a phosphoglucomutase, a glucose-1-phosphatase, a glucose-1-phosphate adenylyltransferase, or a combination thereof.
A metabolically engineered E. coli or S. cerevisiae for the production of UDP-glucose, wherein said metabolically engineered E. coli or S. cerevisiae has been genetically modified by introducing a heterologous gene encoding a sucrose phosphorylase capable of splitting sucrose into glucose-1-phosphate and fructose, comprises a UDP-glucose pyrophosphorylase, and has been further genetically modified to disrupt an endogenous gene encoding a glucose-1-phosphatase, a phosphoglucomutase, and/or a glucose-1-phosphate adenylyltransferase.
A metabolically engineered E. coli or S. cerevisiae for the production of glucose-6-phosphate, wherein said metabolically engineered E. coli or S. cerevisiae has been genetically modified by introducing a heterologous gene encoding a sucrose phosphorylase capable of splitting sucrose into glucose-1-phosphate and fructose, comprises a phosphoglucomutase, and has been further genetically modified to disrupt an endogenous gene encoding a glucose-1-phosphatase and/or a glucose-1-phosphate adenylyltransferase.
A metabolically engineered E. coli or S. cerevisiae for the production of glucose-1-phosphate, wherein said metabolically engineered E. coli or S. cerevisiae has been genetically modified by introducing a heterologous gene encoding a sucrose phosphorylase capable of splitting sucrose into glucose-1-phosphate and fructose, and has been further genetically modified to disrupt an endogenous gene encoding a glucose-1-phosphatase, a phosphoglucomutase, and/or a glucose-1-phosphate adenylyltransferase.
Dependencies are inferred from the claim text.