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US 11,384,352 B2Granted
RNA affinity purification
AssigneeModernaTX, Inc.
InventorEdward J. Miracco
Life of this patent
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Abstract
Provided herein, in some embodiments, are methods of purifying a nucleic acid preparation. The methods may comprise contacting a nucleic acid preparation comprising messenger ribonucleic acid with an RNase III enzyme that is immobilized on a solid support and binds to double-stranded RNA contaminants.
Claims18
A method of purifying a nucleic acid preparation, comprising:
- contacting a nucleic acid preparation comprising messenger ribonucleic acid (mRNA) and double stranded RNA (dsRNA) with an RNase III enzyme that (i) is immobilized on a solid support, (ii) is catalytically inactive, and (iii) can specifically bind to the dsRNA;
- and
- eluting a purified nucleic acid preparation comprising the mRNA from the solid support.
An affinity purification method comprising:
- passing a nucleic acid preparation over an affinity purification column to which an RNase III enzyme is immobilized, wherein the nucleic acid preparation comprises messenger ribonucleic acid (mRNA) and double stranded RNA (dsRNA), and wherein the RNase III enzyme is catalytically inactive and can specifically bind to the dsRNA;
- and
- eluting a purified nucleic acid preparation comprising the mRNA from the solid support.
Dependencies are inferred from the claim text.