Serine proteases of the Bacillus gibsonii-clade
AssigneeDanisco US Inc.
InventorsMarc Kolkman, Frits Goedegebuur, Lilia Maria Babe, Rie Mejldal, Harm Mulder, Anja Hemmingsen Kellett-Smith, Richard R Bott, Miles Christopher Scotcher
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Abstract
The present disclosure relates to serine proteases cloned from Bacillus gibsonii, and variants thereof. Compositions containing the serine proteases are suitable for use in cleaning fabrics and hard surfaces, as well as in a variety of industrial applications.
Claims17
A recombinant polypeptide or an active fragment thereof of the Bacillus Gibsonii-clade, wherein the recombinant polypeptide or active fragment thereof has proteolytic activity and comprises an amino acid sequence having at least 97% identity to the amino acid sequence of SEQ ID NO:
- 69, wherein said Bacillus Gibsonii-clade comprises a DXGIXXHSDLXXXGGASXXXXXPTTADLNXHGTH (SEQ ID NO:47) or DXGIXXHSDLXXXGGASXXXXXXTTADLXXHGTH (SEQ ID NO:90) motif, wherein the initial D is the active site Aspartic acid residue and the penultimate H is the active site Histidine, and X is any amino acid.
The recombinant polypeptide or active fragment thereof of any preceding claim, wherein the polypeptide retains at least 50% of its maximal protease activity at a pH range of 8 to 12 or a temperature range of 50° C. to 75° C.
A polynucleotide comprising a nucleic acid sequence:
- (i) encoding an amino acid sequence of SEQ ID NO:47 or 49;
- (ii) encoding an amino acid sequence of SEQ ID NO:47 or 49 and further encoding an amino acid sequence having 97% identity to an amino acid sequence of SEQ ID NO:
- (iii) encoding an amino acid sequence having 70% identity to an amino acid sequence of SEQ ID NO:
- (iv) having at least 90% identity to SEQ ID NO:
- or
- (xi) having complementarity to SEQ ID NO:.
Dependencies are inferred from the claim text.