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US 11,384,342 B2Granted
Selection marker for cell transfection and protein production
AssigneeSanofi
InventorsBruno Dumas, Nabil Lounis
Life of this patent
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Abstract
The present invention is within the field of industrial protein production. The invention provides a novel expression system using dehydroorotate dehydrogenase (DHODH) as a selection marker in combination with leflunomide or a metabolite thereof, notably for use in mammalian cell lines. Expression vectors encoding DHODH, cell lines comprising said vectors and methods of producing recombinant proteins are also provided.
Claims5
An expression system comprising
- (a) an expression vector comprising a nucleotide sequence encoding a mammalian dihydroorotate dehydrogenase (DHODH) and at least one expression cassette for expressing a recombinant protein, wherein the DHODH comprises the nucleotide sequence of SEQ ID NO:
- 1 or a nucleotide sequence encoding an amino acid sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO:
- 2, wherein the DHODH is inhibited by a DHODH inhibitor, and wherein the DHODH inhibitor is teriflunomide;
- and
- (b) at least one DHODH inhibitor, wherein the at least one DHODH inhibitor is teriflunomide.
2 more elements
Dependencies are inferred from the claim text.