Debriding composition for treating wounds
AssigneeMediWound Ltd.
InventorsEilon Asculai, Dafna Geblinger, Mery Kleyman, Deborah Hanah Bartfeld
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Abstract
The present invention relates to debriding compositions in the form of an aqueous gel. Particularly, the present invention relates to a debriding composition comprising a proteolytic enzyme mixture obtained from bromelain present in a dry form, and an aqueous gel carrier, wherein, prior to use, the proteolytic enzyme mixture being admixed with the aqueous gel carrier to form a debriding composition useful for debridement and treatment of c wounds.
Claims16
A method for debridement of a chronic or hard to heal wound comprising a step of applying to a site of the chronic or hard to heal wound in a subject in need of such treatment a therapeutically effective amount of a debriding composition comprising:
- (a) a proteolytic enzyme mixture obtained from bromelain comprising stem bromelain (EC 3.4.22.32), ananain (EC 3.4.22.31), a cysteine protease precursor, and jacalin-type lectin, the proteolytic enzyme mixture being in a dry or lyophilized form;
- (b) a pH adjusting agent;
- and
- (c) a carrier comprising:
- (i) a carbomer present in an amount of about 0.9% (w/w) of the total weight of the carrier;
- (ii) a polar co-solvent which is glycerol present in an amount of about 10% (w/w) of the total weight of the carrier;
- and
- (iii) water,
- wherein the proteolytic enzyme mixture (a) is admixed with the pH adjusting agent (b) and the carrier (c) to form the debriding composition in the form of a gel that has a viscosity in the range of about 15,000 centipoise (cP) to about 25,000 cP and a pH ranging from about 6.0 to about 8.0,
- wherein said proteolytic enzyme mixture is present in the debriding composition in an amount ranging from about 1% (w/w) to about 5% (w/w) of the total weight of the carrier,
- wherein the step of applying said debriding composition is performed in a regimen of two applications to up to ten applications, and
- wherein the debriding composition is maintained in contact with the wound site for at least about 4 hours to about 72 hours per application.
A method for debridement of a chronic or hard to heal wound comprising a step of applying to a site of the chronic or hard to heal wound in a subject in need of such treatment a therapeutically effective amount of a debriding composition comprising:
- (a) a proteolytic enzyme mixture obtained from bromelain comprising stem bromelain (EC 3.4.22.32), ananain (EC 3.4.22.31), a cysteine protease precursor, and jacalin-type lectin, the proteolytic enzyme mixture being in a dry or lyophilized form;
- (b) a carrier comprising:
- (i) a carbomer present in an amount of about 0.9% (w/w) of the total weight of the carrier;
- (ii) a polar co-solvent which is glycerol present in an amount of about 10% (w/w) of the total weight of the carrier;
- and
- (iii) a pH adjusting agent which is anhydrous di-sodium hydrogen phosphate present in an amount of about 0.25% (w/w) of the total weight of the carrier;
- and
- (iv) water,
- wherein the proteolytic enzyme mixture (a) is admixed with the carrier (b) to form the debriding composition in the form of a gel that has a viscosity ranging from about 15,000 cP to about 25,000 cP and a pH ranging from about 6.0 to about 7.0,
- wherein said proteolytic enzyme mixture is present in an amount ranging from about 1% (w/w) to about 5% (w/w) of the total weight of the carrier,
- wherein the step of applying said debriding composition is performed in a regimen of two applications to up to ten applications, and
- wherein the debriding composition is maintained in contact with the wound site for at least about 4 hours to about 72 hours per application.
A method for complete debridement and/or wound closure of a chronic or hard to heal wound comprising a step of applying to a site of the chronic or hard to heal wound in a subject in need of such treatment a therapeutically effective amount of a debriding composition comprising:
- (a) a proteolytic enzyme mixture obtained from bromelain comprising stem bromelain (EC 3.4.22.32), ananain (EC 3.4.22.31), a cysteine protease precursor, and jacalin-type lectin, the proteolytic enzyme mixture being in a dry or lyophilized form;
- (b) a pH adjusting agent;
- and
- (c) a carrier comprising:
- (i) a carbomer present in an amount of about 0.9%_(w/w) of the total weight of the carrier;
- (ii) a polar co-solvent which is glycerol present in an amount of about 10% (w/w) of the total weight of the carrier;
- and
- (iii) water,
- wherein the proteolytic enzyme mixture (a) is admixed with the pH adjusting agent (b) and the carrier (c) to form the debriding composition in the form of a gel that has a viscosity in the range of about 15,000 centipoise (cP) to about 25,000 cP and a pH ranging from about 6.0 to about 8.0,
- wherein said proteolytic enzyme mixture is present in the debriding composition in an amount ranging from about 1% (w/w) to about 5% (w/w) of the total weight of the carrier,
- wherein the step of applying said debriding composition is performed in a regimen of two applications to up to ten applications, with the debriding composition maintained in contact with the wound site for at least about 4 hours to about 72 hours per application with the number of applications and time of contact of the debriding composition with the wound site conducted to achieve complete wound debridement and/or wound closure.
Dependencies are inferred from the claim text.