US 11,378,580 B2 · Protein detection method using mass spectrometry

Abstract

The present invention provides: a method of pretreating a serum or plasma sample for detection of a protein or a plurality of proteins of interest in a serum or plasma sample via mass spectrometry and a method of detecting such a protein or proteins, wherein proteins such as albumin present in abundance in a sample are removed in a convenient manner, thereby making it possible to collect digested peptides from the protein of interest. Specifically, the present invention provides a method of pretreating a sample for detecting proteins in a serum or plasma sample via mass spectrometry, comprising a step of adding a protease to the sample under non-denaturing conditions to digest proteins and a step of separating the obtained peptides from undigested proteins, and a method of detecting proteins, comprising subjecting the obtained peptides to mass spectrometry.

Claims19

Dependent claims

A method of detecting a target protein in a serum or plasma sample via mass spectrometry, the method comprising:

(a) directly adding a protease to the sample including albumin and the target protein under a temperature in a range of 4° C. to 37° C. and a pH in a range of 7 to 9, thereby digesting the target protein while the albumin is not digested to obtain a mixture containing the albumin and peptides derived from the target protein;
(b) removing the albumin from the mixture of (a);
and
(c) subjecting the mixture after the removal of the albumin to mass spectrometry, wherein the method excludes immunoprecipitation and ion-exchange chromatography.

1 more element

Dependencies are inferred from the claim text.

Protein detection method using mass spectrometry

Life of this patent

Protein detection method using mass spectrometry

Life of this patent

Abstract

Claims19