Image processing device, imaging system, actuation method of image processing device, and computer-readable recording medium

An actuation method of an image processing device, the actuation method comprising:

• with respect to each pixel included in a fluorescent image obtained by capturing a tissue group including one or more tissues dyed by using a fluorescent dye group including one or more fluorescent dyes by a fluorescent microscope, estimating a light quantity of fluorescence emitted by each fluorescent dye of the fluorescent dye group;
• estimating a tissue amount of each tissue of the tissue group based on the estimated light quantity;
• estimating a virtual dye amount of each dye of a bright-field dye group of a case in which the tissue group dyed by using the bright-field dye group including one or more dyes is captured by a transmission-type microscope based on the estimated tissue amount;
• and
• generating a virtual transmitted-light image from the estimated dye amount.

A non-transitory computer-readable recording medium with an executable program stored thereon, the program causing an image processing device to execute:

• with respect to each pixel included in a fluorescent image obtained by capturing a tissue group including one or more tissues dyed by using a fluorescent dye group including one or more fluorescent dyes by a fluorescent microscope, estimating a light quantity of fluorescence emitted by each fluorescent dye of the fluorescent dye group;
• estimating a tissue amount of each tissue of the tissue group based on the estimated light quantity;
• estimating a virtual dye amount of each dye of a bright-field dye group of a case in which the tissue group dyed by using the bright-field dye group including one or more dyes is captured by a transmission-type microscope based on the estimated tissue amount;
• and
• generating a virtual transmitted-light image from the estimated dye amount.