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US 11,377,688 B2Granted
Compositions and methods for detecting C1orf43 nucleic acid
AssigneeGen-Probe Incorporated
InventorsSiobhán M. Miick, Paul M. Darby, Jo Ann Jackson, Damon Kittredge Getman
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Abstract
This disclosure provides oligomers, combinations of oligomers, compositions, kits, uses, and methods for detecting a C1orf43 nucleic acid, such as C1orf43 mRNA, such as human C1orf43 mRNA, in a sample.
Claims21
A method of detecting the presence or absence of a C1orf43 nucleic acid in a sample, comprising:
- contacting the sample with a combination of oligomers comprising at least first and second amplification oligomers,
- performing a nucleic acid amplification reaction which produces at least a first amplicon in the presence of the C1orf43 nucleic acid,
- and detecting the presence or absence of the first amplicon,
- wherein:
- the first amplicon is produced through extension of the first and second amplification oligomers in the presence of the C1orf43 nucleic acid;
- and
- wherein the first and second amplification oligomers are reverse and forward amplification oligomers, respectively;
- and are configured to
- specifically hybridize to first and second sites in the sequence of SEQ ID NO:
- 39, respectively, wherein the first site is in the complement of SEQ ID NO:
- 52 and the first amplification oligomer comprises the sequence of SEQ ID NO:53 or SEQ ID NO:54;
- and
- wherein the second site is in the complement of SEQ ID NO:
- 46 and the second amplification oligomer comprises the sequence of SEQ ID NO:
- 47 or SEQ ID NO:48.
12 more elements
A method of isolating C1orf43 nucleic acid from a sample, comprising:
- contacting the sample with at least one capture oligomer under conditions permissive for forming one or more complexes of a capture oligomer and the C1orf43 nucleic acid, thereby forming a composition, wherein the capture oligomer comprises a target hybridizing sequence comprising the sequence of SEQ ID NO:
- 56 or SEQ ID NO:57 and is configured to specifically hybridize to a capture site in the sequence of SEQ ID NO:
- 55;
- and
- isolating the capture oligomer from the composition.
2 more elements
Dependencies are inferred from the claim text.