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US 11,377,683 B2Granted
Looped primer and loop-de-loop method for detecting target nucleic acid
AssigneeUh-Oh Labs Inc.
InventorCameron Scott Ball
Life of this patent
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Abstract
The present disclosure provides a novel loop-de-loop method of detecting a target nucleic acid using a biosensor-labeled oligonucleotide. Further provided herein is a looped primer and a kit for use in the method.
Claims29
A looped primer for loop-de-loop amplification (LdL) of a target sequence, comprising from 5′ to 3′:
- a first sensor molecule;
- a first clamping oligonucleotide;
- a spacing oligonucleotide;
- a second clamping oligonucleotide,
- wherein the first clamping oligonucleotide, the spacing oligonucleotide and the second clamping oligonucleotide can form a hairpin structure at a temperature below the melting temperature (Tm) of the first and second clamping oligonucleotides;
- a second sensor molecule,
- wherein the first sensor molecule and the second sensor molecule are a first biosensor pair;
- and
- a first primer sequence complementary to a first binding site on the target sequence;
- and
- further comprising at least a first additional oligonucleotide, wherein the first additional oligonucleotide is at 5′ end of the looped primer.
8 more elements
Dependencies are inferred from the claim text.