Compositions and methods for nucleic acid amplification

A method, comprising:

• (a) providing a reaction mixture comprising:
• (i) a single-stranded target DNA molecule comprising a single nucleotide polymorphism (SNP) site and
• (ii) a probe, wherein said probe has (1) a known DNA sequence (2) at least one cleavable moiety, (3) a detectable moiety, and (4) a quencher, wherein said probe is complementary to said single-stranded target DNA molecule, and wherein said quencher quenches said detectable moiety when said probe is not hybridized to said single-stranded target DNA molecule;
• wherein the probe is not capable of intramolecular hybridization;
• (b) hybridizing said probe to said single-stranded target DNA molecule such that, said cleavable moiety is situated at the SNP site of said single-stranded target DNA molecule, wherein complementarity of the cleavable moiety with a nucleotide of the SNP site allows cleavage of the cleavable moiety and separation of the quencher from the detectable moiety;
• and
• (c) detecting a signal from said probe that is indicative of a presence of said nucleotide in said SNP site;
• wherein (b) and (c) are performed without the aid of an enzyme;
• wherein the method is configured to detect the SNP in a target DNA.