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US 11,377,680 B2Granted
Linkers and methods for optical detection and sequencing
AssigneeUltima Genomics, Inc.
InventorsLinda G. Lee, Gilad Almogy, Steven Menchen
Life of this patent
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Abstract
The present disclosure provides labeling reagents for labeling substrates such as nucleotides, proteins, antibodies, lipids, and cells. The labeling reagents provided herein may comprise fluorescent labels and semi-rigid linkers. Methods for nucleic acid sequencing using materials comprising such labeling reagents are also provided here.
Claims23
A method, comprising:
- (a) contacting a nucleic acid molecule with a solution comprising a plurality of non-terminated nucleotides under conditions sufficient to incorporate, in succession, a first nucleotide and a second nucleotide of said plurality of non-terminated nucleotides into a growing strand that is complementary to said nucleic acid molecule, wherein said first nucleotide is labeled, and wherein at least about 20% of said plurality of non-terminated nucleotides are labeled nucleotides;
- (b) detecting one or more signals or signal changes from said first nucleotide, wherein said one or more signals or signal changes are indicative of incorporation of said first nucleotide;
- and
- (c) resolving said one or more signals or signal changes to determine a sequence of said nucleic acid molecule.
1 more element
Dependencies are inferred from the claim text.