Fermentation process
AssigneeLonza Ltd.
InventorsWieland Reichelt, Christoph Herwig, Julian Kager, Patrick Sagmeister, Matthias Funke
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Abstract
A method for producing a recombinant protein of interest (POI) is provided, comprising the steps of (a) culturing cells in a cell culture medium to express said POI by adding a feed comprising at least one substrate to said cell culture, (b) applying a feeding strategy based on calculating, setting and optionally controlling the specific substrate uptake rate qs of the cells during the induction phase and/or production phase of the POI, wherein qs is set to be close to the maintenance rate of the cell culture; and (c) isolating said POI from the cell culture.
Claims18
A method for producing a recombinant protein of interest (POI) comprising:
- (a) providing a regulatable promoter operably linked to a nucleic acid encoding the POI in prokaryotic cells or yeast cells;
- (b) culturing the cells in a cell culture medium that comprises at least one carbohydrate as the substrate carbon source;
- (c) setting the specific carbohydrate substrate uptake rate qs of the cells to be close to the maintenance rate of the cell culture during the expression phase of the POT, wherein said qs is in the range of 0.03 to 0.15 g/g/h;
- (d) expressing the POI in the prokaryotic cells or yeast cells;
- and
- (e) isolating the POI from the cell culture.
A method for producing a recombinant protein of interest (POI) comprising:
- (a) culturing procaryotic or yeast cells in a cell culture medium to express said POI by adding a feed comprising at least one carbohydrate as the carbon source to said cell culture, wherein the POI is expressed using an expression system or expression cassette comprising a regulatable promoter operably linked to the nucleic acid encoding the POI,
- (b) setting the specific carbohydrate substrate uptake rate qs to be slightly above the maintenance rate of the cell culture, wherein said qs is in the range of 0.03 to 0.15 g/g/h;
- and
- (c) isolating said POI from the cell culture.
Dependencies are inferred from the claim text.