Co-production pathway for 3-HPA and acetyl-CoA derivatives from malonate semialdehyde
AssigneeBraskem S.A.
InventorsBeatriz Leite Magalhaes, Paulo Moises Raduan Alexandrino, Felipe Galzerani
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Abstract
The present disclosure provides methods for utilizing genetically modified microbes to co-produce 3-hydroxypropionic acid (3-HP) and acetyl-CoA, and derivatives thereof from malonate semialdehyde as a common single intermediate. The disclosure further provides modified microbe that co-produce the 3-HP and acetyl-CoA derivatives from malonate semialdehyde.
Claims19
A recombinant microorganism capable of co-producing 3-hydroxypropionic acid (3-HP), and/or derivatives thereof, and acetyl-CoA from a feedstock comprising one or more carbon sources, wherein the recombinant microorganism comprises:
- (a) at least one exogenous nucleic acid molecule encoding an enzyme that is able to catalyze the decarboxylation of oxaloacetate into malonate semialdehyde;
- (b) at least one endogenous and/or exogenous nucleic acid molecule encoding a 3-hydroxypropionic acid dehydrogenase that catalyzes the production of 3-HP from malonate semialdehyde from (a);
- and
- (c) at least one endogenous and/or exogenous nucleic acid molecule encoding a
- (i) malonate semialdehyde dehydrogenase that catalyzes the production of acetyl-CoA from malonate semialdehyde from (a), and/or
- (ii) malonyl-CoA reductase and/or 2-keto acid decarboxylase that catalyzes the conversion of malonate semialdehyde from (a) into malonyl-CoA, and malonyl-CoA decarboxylase that catalyzes the production of acetyl-CoA from malonyl-CoA;
- wherein at least a portion of excess NAD(P)H generated in the production of acetyl-CoA is utilized as a source of reducing equivalents in the production of 3-HP and/or 3-HP derivatives;
- wherein the derivatives of 3-HP are selected from the group consisting of:
- acrylic acid, 1-propanol, propene, and polypropylene.
Dependencies are inferred from the claim text.