Compositions and methods comprising male fertility sequences
AssigneePioneer Hi-Bred International, Inc.
InventorsMarc C. Albertsen, Andrew Mark Cigan, Howard P. Hershey, Michael Lassner, Yongzhong Wu
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Abstract
Compositions and methods for modulating male fertility in a plant are provided. Compositions comprise nucleotide sequences, and active fragments and variants thereof, which modulate male fertility. Further provided are expression cassettes comprising the male fertility polynucleotides, or active fragments or variants thereof, operably linked to a promoter, wherein expression of the polynucleotides modulates the male fertility of a plant. Various methods are provided wherein the level and/or activity of the sequences that influence male fertility is modulated in a plant or plant part.
Claims14
A construct comprising an expression cassette, wherein said expression cassette comprises a first promoter that drives expression of an operably-linked first polynucleotide in a wheat plant, wherein said first promoter is a male tissue-preferred promoter heterologous to the operably linked first polynucleotide, and wherein the operably-linked first polynucleotide is an Ms26 polynucleotide that has a nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence comprising SEQ ID NO:
- 7;
- (b) a nucleotide sequence encoding a polypeptide comprising SEQ ID NO:
- 8, wherein said polypeptide increases male fertility in a mutated male-sterile Ms26 wheat plant;
- (c) a nucleotide sequence comprising at least 90% sequence identity to the full length sequence of SEQ ID NO:
- 7, wherein said nucleotide sequence encodes a polypeptide that increases male fertility in a mutated Ms26 male-sterile wheat plant;
- and
- (d) a nucleotide sequence encoding a polypeptide having at least 95% sequence identity to the full length of the polypeptide sequence of SEQ ID NO:
- 8, wherein said polypeptide increases male fertility in a mutated Ms26 male-sterile wheat plant.
A method of increasing male fertility in a mutated Ms26 male sterile wheat plant, relative to a control plant, wherein said method comprises introducing into said wheat plant a polynucleotide operably linked to a male tissue-preferred promoter heterologous to the polynucleotide, wherein said promoter drives expression in the wheat plant, said polynucleotide comprising a nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence comprising SEQ ID NO:
- 7;
- (b) a nucleotide sequence encoding a polypeptide comprising SEQ ID NO:
- 8, wherein said polypeptide increases male fertility in a mutated male-sterile Ms26 wheat plant;
- (c) a nucleotide sequence comprising at least 90% sequence identity to the full length sequence of SEQ ID NO:
- 7, wherein said nucleotide sequence encodes a polypeptide that increases male fertility in a mutated Ms26 male-sterile wheat plant;
- and
- (d) a nucleotide sequence encoding a polypeptide having at least 95% sequence identity to the full length of the polypeptide sequence of SEQ ID NO:
- 8, wherein said polypeptide increases male fertility in a mutated Ms26 male-sterile wheat plant, and wherein expression of the polynucleotide increases the wheat plant's male fertility.
Dependencies are inferred from the claim text.