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US 11,377,667 B2Granted
Compositions and methods for controlling Leptinotarsa
AssigneeMonsanto Technology LLC
InventorsJodi Lynn Beattie, Michael John Crawford, Brian Donovan Eads, Lex Evan Flagel, Mahak Kapoor, Christina Marie Taylor
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Abstract
Disclosed herein are methods of controlling insect pests, in particular Leptinotarsa spp. which infest crop plants, and methods of providing plants resistant to such pests. Also disclosed are polynucleotides and recombinant DNA molecules and constructs useful in such methods, insecticidal compositions such as topical sprays containing insecticidal double-stranded RNAs, and solanaceous plants with improved resistance to infestation by Leptinotarsa spp. Further disclosed are methods of selecting target genes for RNAi-mediated silencing and control of Leptinotarsa spp.
Claims18
A method for controlling a Leptinotarsa species infestation of a plant comprising:
- (a) contacting said Leptinotarsa species with a polynucleotide comprising an inhibitory nucleotide sequence that is complementary to at least 21 contiguous nucleotides of SEQ ID NO:807, or an RNA transcribed therefrom;
- or
- (b) providing in the diet of said Leptinotarsa species a polynucleotide comprising an inhibitory nucleotide sequence that is complementary to at least 21 contiguous nucleotides of SEQ ID NO:807, or an RNA transcribed therefrom;
- or
- (c) causing mortality or stunting in larvae of said Leptinotarsa species by providing in the diet of said larvae at least one polynucleotide comprising at least one silencing element comprising 21 contiguous nucleotides that are complementary to SEQ ID NO:807, or an RNA transcribed therefrom;
- or
- (d) topically applying to said plant a composition comprising at least one polynucleotide comprising an inhibitory nucleotide sequence that is complementary to at least 21 contiguous nucleotides of SEQ ID NO:807, or an RNA transcribed therefrom;
- or
- (e) topically applying to said plant an inhibitory composition comprising at least one polynucleotide in a manner such that an effective amount of said polynucleotide is ingested by Leptinotarsa species feeding on said plant, said polynucleotide comprising at least 21 contiguous nucleotides that are complementary to SEQ ID NO:807, or an RNA transcribed therefrom;
- or
- (f) expressing in said plant at least one polynucleotide comprising at least one inhibitory segment that is identical or complementary to at least 21 contiguous nucleotides of SEQ ID NO:807;
- or
- (g) providing to said plant at least one polynucleotide comprising at least one inhibitory segment that is identical or complementary to at least 21 contiguous nucleotides of SEQ ID NO:
- 807 or an RNA transcribed therefrom;
- or
- (h) contacting said Leptinotarsa species with a polynucleotide comprising at least one inhibitory segment that is identical or complementary to at least 21 contiguous nucleotides of SEQ ID NO:
- 807, or an RNA transcribed therefrom.
14 more elements
An insecticidal composition for controlling a Leptinotarsa species, comprising:
- (a) an insecticidally effective amount of a polynucleotide comprising at least 21 contiguous nucleotides that are complementary to a SEQ ID NO:807, or an RNA transcribed therefrom;
- or
- (b) an insecticidally effective amount of at least one polynucleotide comprising at least one silencing element that is complementary to at least 21 contiguous nucleotides of a target gene or an RNA transcribed from said target gene, wherein said target gene has a nucleotide sequence of SEQ ID NO:807;
- or
- (c) an insecticidally effective amount of at least one RNA comprising at least one segment that is identical or complementary to at least 21 contiguous nucleotides of SEQ ID NO:807, or an RNA transcribed therefrom;
- or
- (d) an RNA molecule that causes mortality or stunting of growth in a Leptinotarsa species when ingested or contacted by said Leptinotarsa species, wherein said RNA molecule comprises at least 21 contiguous nucleotides that are complementary to SEQ ID NO:807, or an RNA transcribed therefrom;
- or
- (e) an insecticidal double-stranded RNA molecule that causes mortality or stunting of growth in a Leptinotarsa species when ingested or contacted by said Leptinotarsa species, wherein at least one strand of said insecticidal double-stranded RNA molecule comprises 21 contiguous nucleotides that are complementary to a target gene or an RNA transcribed from said target gene, wherein said target gene has a sequence of SEQ ID NO:807;
- or
- (f) an insecticidally effective amount of at least one double-stranded RNA comprising SEQ ID NO:
- 1049.
9 more elements
A recombinant DNA construct comprising a heterologous promoter operably linked to:
- (a) DNA comprising a nucleotide sequence that is complementary to at least 21 contiguous nucleotides of a target gene having a sequence of SEQ ID NO:807, or an RNA transcribed therefrom;
- or
- (b) a DNA comprising 21 or more contiguous nucleotides having 100% identity to a fragment of equivalent length of a DNA having a sequence of SEQ ID NO:807, or the DNA complement thereof;
- or
- (c) DNA encoding at least one silencing element that is complementary to at least 21 contiguous nucleotides of a target gene or an RNA transcribed from said target gene, wherein said target gene has a sequence of SEQ ID NO:807;
- or
- (d) DNA encoding at least one silencing element comprising at least 21 contiguous nucleotides that are complementary to a target gene of SEQ ID NO:
- 807, or an RNA transcribed therefrom;
- or
- (e) DNA encoding a RNA comprising at least 21 contiguous nucleotides that are complementary to SEQ ID NO:
- 1049, or the complement thereof, or an orthologous nucleotide sequence from a Leptinotarsa species or a Tribolium species, wherein the orthologous nucleotide sequence has at least 95% sequence identity with SEQ ID NO:
- 1049, wherein the percentage sequence identity is calculated over the same length;
- or
- (f) DNA encoding a RNA comprising at least one double-stranded RNA region, at least one strand of which comprises at least 21 contiguous nucleotides that are complementary to SEQ ID NO:
- 1049, or the complement thereof, or an orthologous nucleotide sequence from a Leptinotarsa species or a Tribolium species, wherein the orthologous nucleotide sequence has at least 95% sequence identity with SEQ ID NO:
- 1049, wherein the percentage sequence identity is calculated over the same length;
- or
- (g) DNA encoding RNA comprising SEQ ID NO:
- 1049, or the complement thereof.
16 more elements
Dependencies are inferred from the claim text.