US 11,377,665 B2Granted

Composition and methods for producing tobacco plants and products having increased phenylalanine and reduced tobacco-specific nitrosamines (TSNAs)

AssigneeAltria Client Services LLC

InventorsChengalrayan Kudithipudi, Dongmei Xu, James Strickland, Yanxin Shen

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Abstract

The present disclosure provides approaches for reducing tobacco-specific nitrosamines (TSNAs) in tobacco. Some of these approaches include genetically engineering tobacco plants to increase one or more antioxidants, increase oxygen radicle absorbance capacity (ORAC), increase phenylalanine, or reduce nitrite. Also provided are methods and compositions for producing modified tobacco plants and tobacco products therefrom comprising reduced TSNAs. Also provided are methods and compositions for increasing the expression of chorismate mutase and other transcription factors involved in anthocyanin biosynthesis.

Claims12

Dependent claims

A cured tobacco leaf of a modified tobacco plant, wherein said cured tobacco leaf comprises a decreased amount of one or more tobacco specific nitrosamines (TSNAs), a recombinant nucleic acid molecule comprising a promoter operably linked to a first polynucleotide encoding a Chorismate Mutase-like polypeptide having at least 95% sequence identity to a sequence selected from the group consisting of SEQ ID NOs:

68 to 70, and a recombinant nucleic acid molecule comprising a promoter operably linked to a second polynucleotide encoding a MYB3-like transcription factor polypeptide having at least 95% sequence identity to a sequence selected from the group consisting of SEQ ID NOs:
13 and 23, wherein said decreased amount is compared to an unmodified control tobacco plant.

Dependencies are inferred from the claim text.

Facts

Application: 16/372,833Filed 2 Apr 2019 · us provisional application
Priority: 3 Apr 2018
Published: US 2019/0300893 A13 Oct 2019
Granted: 5 Jul 2022
Term ends: Not estimatedThe source records don’t support an estimate.

Classification15 codes

C12N 15/8243
With non-agronomic quality traits, e.g. for industrial processing; Value added, non-agronomic traits: involving biosynthetic or metabolic…
A24B 15/20
Treatment of tobacco products or tobacco substitutes: biochemical treatment
A24B 15/245
Extraction of specific substances: nitrosamines
C12N 9/88
Enzymes; Proenzymes; Compositions thereof ; Processes for preparing, activating, inhibiting, separating or purifying enzymes: lyases